2X SYBR Green qPCR Master Mix
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Quantitative PCR (qPCR, also called Real-time PCR), is a popular technology for precise analysis of gene expression. It can be classified into two categories according to different methods, dye-based and probe-based, in which, dye-based method is more popular, convenient and less costly. Dye-based qPCR monitors real-time fluorescence of the dye binding to the double-stranded DNA to measure DNA amplification indirectly during each cycle. At a point where the fluorescence signal is distinctly detected over the background, Ct value (Cq value) can be determined. The obtained Ct values can be used to evaluate relative target abundance, or calculate absolute target quantities in reference to an appropriate standard curve. Our product 2X SYBR Green qPCR Master Mix provides superior specificity, robust amplification efficiency, ideal reproducibility and stability in quantifying target DNA or cDNA. It’s a 2X PreMix, taking advantage of a hot-start Taq polymerase combined with the antibody. Ideal Taq polymerase and suitable buffer will guarantee preferable specificity and high amplification speed. SYBR Green I in the mix which is a DNA intercalator will emit fluorescence when bounds to the double-stranded DNA amplified in each cycle and monitoring the fluorescence by the instrument allows for the indirect quantification of amplification products at real time. This reagent is supplied together with two ROX Reference Dye of different concentration which should be used to normalize the fluorescent signal intensity between reactions for instruments with the corresponding requirements. When conducting the experiment, use the SYBR® or SYBR/FAM scan mode setting on the real-time instrument.
Quality Control & MSDS
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Related Biological Data
Components and Storage
1000 rxn with 10μL reaction
500 rxn with 20μL reaction
200 rxn with 50μL reaction
5000 rxn with 10μL reaction
2500 rxn with 20μL reaction
1000 rxn with 50μL reaction
10000 rxn with 10μL reaction
5000 rxn with 20μL reaction
2000 rxn with 50μL reaction
|2X SYBR Green qPCR Master Mix|
|50X ROX Reference Dye (low concentration)|
|50X ROX Reference Dye (high concentration)|
Store all components at -20°C and protect from light. Avoid repeated freeze/thaw cycles as possible.
Hot-start mechanism for optimized specificity
Superior sensitivity and accuracy
Highly reproducible Ct values over a broad dynamic range
Convenient 2X PreMix formats to simplify experimental steps