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2X SYBR Green qPCR Master Mix

Catalog No.
K1070
 
Grouped product items
SizePriceStock Qty
5ml
$120.00
In stock
25ml
$420.00
In stock
50ml
$840.00
In stock

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

Product Citation

Description

Quantitative PCR (qPCR, also called Real-time PCR), is a popular technology for precise analysis of gene expression. It can be classified into two categories according to different methods, dye-based and probe-based, in which, dye-based method is more popular, convenient and less costly. Dye-based qPCR monitors real-time fluorescence of the dye binding to the double-stranded DNA to measure DNA amplification indirectly during each cycle. At a point where the fluorescence signal is distinctly detected over the background, Ct value (Cq value) can be determined. The obtained Ct values can be used to evaluate relative target abundance, or calculate absolute target quantities in reference to an appropriate standard curve. Our product 2X SYBR Green qPCR Master Mix provides superior specificity, robust amplification efficiency, ideal reproducibility and stability in quantifying target DNA or cDNA. It’s a 2X PreMix, taking advantage of a hot-start Taq polymerase combined with the antibody. Ideal Taq polymerase and suitable buffer will guarantee preferable specificity and high amplification speed. SYBR Green I in the mix which is a DNA intercalator will emit fluorescence when bounds to the double-stranded DNA amplified in each cycle and monitoring the fluorescence by the instrument allows for the indirect quantification of amplification products at real time. This reagent is supplied together with two ROX Reference Dye of different concentration which should be used to normalize the fluorescent signal intensity between reactions for instruments with the corresponding requirements. When conducting the experiment, use the SYBR® or SYBR/FAM scan mode setting on the real-time instrument.

Quality Control

Quality Control & MSDS

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Related Biological Data

2X SYBR Green qPCR Master Mix
 

Components and Storage

Components
5mL
1000 rxn with 10μL reaction
500 rxn with 20μL reaction
200 rxn with 50μL reaction
25mL
5000 rxn with 10μL reaction
2500 rxn with 20μL reaction
1000 rxn with 50μL reaction
50mL
10000 rxn with 10μL reaction
5000 rxn with 20μL reaction
2000 rxn with 50μL reaction
2X SYBR Green qPCR Master Mix
1 mL x 5
1 mL x 25
5 mL x 10
50X ROX Reference Dye (low concentration)
0.2 mL
1 mL
1 mL x 2
50X ROX Reference Dye (high concentration)
0.2 mL
1 mL
1 mL x 2

Store all components at -20°C and protect from light. Avoid repeated freeze/thaw cycles as possible.

Features

Hot-start mechanism for optimized specificity

Superior sensitivity and accuracy

Highly reproducible Ct values over a broad dynamic range

Convenient 2X PreMix formats to simplify experimental steps

Storage

Store all components at -20°C and protect from light. Avoid repeated freeze/thaw cycles as possible.