HotStart™ 2X Green qPCR Master Mix

Quantitative PCR (qPCR) employing SYBR Green is widely used to analyze gene expression or quantify nucleic acids, via real-time fluorescence detection. Mechanistically, the SYBR Green dye intercalates into double-stranded DNA, producing fluorescence that corresponds proportionally to amplified DNA amount in each PCR cycle. This approach facilitates indirect, cycle-by-cycle monitoring of DNA amplification, thereby allowing quantification of cDNA or DNA targets. Hot-start methodologies employing antibody-mediated inhibition of Taq polymerase activity until thermal activation further enhance specificity and minimize amplification artifacts. Typically deployed in gene expression analysis, validation of RNA-seq results, or nucleic acid quantitation, HotStart? 2X Green qPCR Master Mix provides benchmark comparisons with conventional PCR mixes, specifically conferring improvements in reaction specificity, reproducibility, and amplification consistency.

Hot-start mechanism for optimized specificity

Superior sensitivity and accuracy

Highly reproducible Ct values over a broad dynamic range

Convenient 2X PreMix formats to simplify experimental steps