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Phos Binding Reagents

Used for Protein Phosphorylation Research.

Phos binding reagents are simple and convenient tools for separation and detection of phosphorylated proteins or peptides.

Protein phosphorylation is an important covalent post-translational modification that can alter the structural conformation of a protein, which then regulates the function, location and specific binding of the target protein. Many cellular processes are regulated by the reversible phosphorylation of proteins and 30% of the proteins are likely to be phosphorylated at some point during their existence.

The determination of the phosphorylation state of proteins is important for defining protein kinase substrates and revealing the activation state of signal transduction pathways. Methods for determining the phosphorylation status of proteins are thus have important implications in the understanding of diverse biological and pathophysiological processes, such as signal transduction pathways, cancer and other diseases.

Phos binding reagents are specific and selective phosphate-binding reagents, and exhibit preferential ionic interactions with phosphorylated ions on phosphorylated proteins or peptides at neutral pH. Phos binding reagent is a dinuclear metal (Zn2+ or Mg2+) complex.

Phos binding reagent acrylamide (PBR-A) is a specific reagent for separation of phosphorylated proteins using SDS-PAGE method. And Phos binding reagent Biotins (PBR-B) are specific reagents for detection of phosphorylated proteins using Western Blot and can instead of the specific phosphorylated antibodies.

  1. Cat.No. Product Name Information
  2. F4002 Phos binding reagent acrylamide An equivalent to Phos-tag acrylamide. Separation of phosphorylated and non-phosphorylated proteins
  3. F4001 Phos binding reagent Biotin An equivalent to Phos-tag Biotin. Detection of phosphorylated proteins
  4. F4004 Phos binding reagent Biotin LC An equivalent to Phos-tag Biotin. Detection of phosphorylated proteins

3 Item(s)

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Basic structure of Phos binding reagents

• <Zn2+-Phos binding reagents>: a stable complex is formed with a phosphate group at the ratio of 1 to 1 at pH 5 ~ 8.

• <Mn2+-Phos binding reagents>: a stable complex is formed with a phosphate group at the ratio of 1 to 1 at around the pH 9.

Features

1. Selective binding phosphate ion(2-).

2. Complex is formed under physiological conditions (pH 5 to 8).

3. Recognition of all phosphorylated forms of Ser / Thr / Tyr.

4. Don't need radioisotope labeling.

5. Followed by mass analysis, etc.

References

1. M. J. Hubbard, P. Cohen. On target with a new mechanism for the regulation of protein phosphorylation. Trends Biochem. Sci. 18: 172 (1993).

2. B. Agnew et al. Compositions and methods for detection and isolation of phosphorylated molecules. US Patent # 7,102,005. (September 5, 2006).