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Molecular Biology
RNA
In Vitro Transcription

In Vitro Transcription

K1100 T4 DNA Ligase (RNase-free)

Summary: T4 DNA ligase, fragments ligation for cohesive or blunt end, nicks repair
K1099 T3 RNA Polymerase (RNase-free)

Summary: DNA-dependent RNA polymerase, highly specific for T3 promoter sequence
K1088 DNase I (RNase-free)

Summary: An endonuclease that can digest single- or double-stranded DNA
K1087 Pyrophosphatase, Inorganic (E. coli)

Application:
Increase the yield of RNA in transcription reaction

DNA Polymerization: Preventing pyrophosphate accumulation

Summary: Pyrophosphatase, inorganic (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to orthophosphates
K1083 T7 RNA Polymerase

Summary: DNA-Dependent RNA Polymerase Specific for T7 Promoter Sequences
K1082 High Yield T7 Biotin16 RNA Labeling Kit
K1068 HyperScribe™ All in One mRNA Synthesis Kit II Plus 2 (EZ Cap Reagent AG (3' OMe), 5-moUTP, T7, poly(A))

Summary: Used for the in vitro synthesis of mRNA (EZ Cap Reagent AG (3' OMe) capped, 5-moUTP modified, poly(A) tailed)
K1067 HyperScribe™ All in One mRNA Synthesis Kit II Plus 1 (EZ Cap Reagent AG (3' OMe), 5mCTP, ψUTP, T7, poly(A))

Summary: Used for the in vitro synthesis of mRNA (EZ Cap Reagent AG (3' OMe) capped, 5mCTP and ψUTP modified, poly(A) tailed)
K1066 HyperScribe™ All in One mRNA Synthesis Kit II (EZ Cap Reagent AG (3' OMe), T7, poly(A))

Summary: Used for the synthesis of EZ Cap Reagent AG (3' OMe) capped and poly(A) tailed mRNA in vitro
K1065 HyperScribe™ All in One mRNA Synthesis Kit Plus 2 (ARCA, 5-moUTP, T7, poly(A))

Summary: Used for the in vitro synthesis of mRNA (ARCA capped, 5-moUTP modified, poly(A) tailed)