T7 RNA Polymerase

This product is a recombinant bacteriophage T7 RNA polymerase expressed in E. coli. It is a DNA-dependent RNA polymerase with high specificity for bacteriophage T7 promoters. The 99 kDa T7 RNA polymerase uses double-stranded DNA containing the T7 promoter sequence as a template, with NTPs as substrates, to synthesize RNA complementary to the single-stranded DNA downstream from the promoter. Double-stranded linear DNA templates with blunt ends or 5' protruding ends can serve as substrates for T7 RNA polymerase. Thus, linearized plasmids or PCR products can be utilized as templates for in vitro transcription of RNA.

RNA produced through T7 RNA polymerase is widely used in many biological experiments, such as in vitro translation, antisense RNA and RNAi experiments, RNA vaccines, studies on RNA structure and function, biochemical analyses involving ribozymes, RNase protection assays, and probe-based hybridization blotting.