T7 RNA Polymerase
This product is a recombinant bacteriophage T7 RNA polymerase expressed in E. coli. It is a DNA-dependent RNA polymerase with high specificity for bacteriophage T7 promoters. The 99 kDa T7 RNA polymerase uses double-stranded DNA containing the T7 promoter sequence as a template, with NTPs as substrates, to synthesize RNA complementary to the single-stranded DNA downstream from the promoter. Double-stranded linear DNA templates with blunt ends or 5' protruding ends can serve as substrates for T7 RNA polymerase. Thus, linearized plasmids or PCR products can be utilized as templates for in vitro transcription of RNA.
RNA produced through T7 RNA polymerase is widely used in many biological experiments, such as in vitro translation, antisense RNA and RNAi experiments, RNA vaccines, studies on RNA structure and function, biochemical analyses involving ribozymes, RNase protection assays, and probe-based hybridization blotting.
- 1. Chaoyang Guan, Yonggeng Ma, et al. "Dual-targets binding protection mediated rolling circle transcription with tandem fluorescent RNA aptamers for label-free detection of liver cancer biomarkers." Sensors and Actuators B: Chemical Volume 408, 1 June 2024, 135521.
- 2. Yue Wang, Yatu Peng, et al. "Co-delivery of Cas9 mRNA and guide RNAs for editing of LGMN gene represses breast cancer cell metastasis." Sci Rep. 2024 Apr 6;14(1):8095. PMID: 38582932
| Components | 5000 U | 25000 U | 100000 U |
| T7 RNA Polymerase (50 U/μL) | 100 μL | 500 μL | 2 mL |
| 10X Reaction Buffer | 100 μL | 500 μL | 2 mL |
Store all the kit components at -20°C. | |||