Cell Counting Kit-8 (CCK-8)
Catalog No.
K1018
Used in cell proliferation assay and cytotoxicity assay
Featured Products
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
- 1. Ning Li, Xiaoping Li, et al. "Inhibition of Sirtuin 3 prevents titanium particle-induced bone resorption and osteoclastsogenesis via suppressing ERK and JNK signaling." Int J Biol Sci. 2021 Apr 3;17(5):1382-1394. PMID:33867853
- 2. Xiangli Zhuang, Bo Wu, et al. "Effect of Xingbi Gel Nasal Drops on Fyn-STAT5 Pathway in Nasal Mucosa Fibroblasts of Guinea Pigs with Allergic Rhinitis." Evid Based Complement Alternat Med. 2021 Mar 20;2021:6686815. PMID:33824677
- 3. Rui-Qi Wang, Xiao-Ran Long, et al. "Lnc-GAN1 expression is associated with good survival and suppresses tumor progression by sponging mir-26a-5p to activate PTEN signaling in non-small cell lung cancer." J Exp Clin Cancer Res. 2021 Jan 6;40(1):9. PMID:33407724
- 4. Peng Zhao, Jianhua Cheng, et al. "LncRNA PCAT6 regulates the progression of pituitary adenomas by regulating the miR-139-3p/BRD4 axis." Cancer Cell Int. 2021 Jan 6;21(1):14. PMID:33407504
- 5. John Culver Taylor, Xinsheng Gao, et al. "A type VII secretion system of Streptococcus gallolyticus subsp. gallolyticus contributes to gut colonization and the development of colon tumors." PLoS Pathog. 2021 Jan 6;17(1):e1009182. PMID:33406160
- 6. Guangyu Ji, Wenjuan Zhou, et al. "Melatonin inhibits proliferation and viability and promotes apoptosis in colorectal cancer cells via upregulation of the microRNA-34a/449a cluster." Mol Med Rep. 2021 Mar;23(3):187. PMID:33398374
- 7. Yun-Feng Zheng, Dan-Yang Li, et al. "Comprehensive Comparison of Two Color Varieties of Perillae Folium Using Rapid Resolution Liquid Chromatography Coupled with Quadruple-Time-of-Flight Mass Spectrometry (RRLC-Q/TOF-MS)-Based Metabolic Profile and in Vivo/ in Vitro Anti-Oxidative Activity." J Agric Food Chem. 2020 Dec 9;68(49):14684-14697. PMID:33237758
- 8. Pei-Ting Lin, Xiao-Dong Xue, et al. "Necrostatin-1, RIP1/RIP3 inhibitor, relieves transforming growth factor β-induced wound-healing process in formation of hypertrophic scars." J Cosmet Dermatol. 2020 Nov 25. PMID:33237588
- 9. Kai Guo, Lei Song, et al. "AEBP1 Promotes Glioblastoma Progression and Activates the Classical NF-κB Pathway." Behav Neurol. 2020 Nov 6;2020:8890452. PMID:33224311
- 10. Zhenyao Chen, Qinnan Chen, et al. "Long non-coding RNA CASC9 promotes gefitinib resistance in NSCLC by epigenetic repression of DUSP1." Cell Death Dis. 2020 Oct 14;11(10):858. PMID:33056982
- 11. Wensi Liu, Zhaojin Yu, et al. "Silencing KIF18B enhances radiosensitivity: identification of a promising therapeutic target in sarcoma." EBioMedicine. 2020 Oct 7;61:103056. PMID:33038765
- 12. Yalei Zhao, Chen Xue, et al. "Comprehensive analysis of ubiquitin‐specific protease 1 reveals its importance in hepatocellular carcinoma." Cell Prolif. 2020 Oct;53(10):e12908. PMID:32951278
- 13. Yingying Gao, Yueling Liu, et al. "Bifunctional Peptide-Conjugated Gold Nanoparticles for Precise and Efficient Nucleus-Targeting Bioimaging in Live Cells." Anal Chem. 2020 Oct 6;92(19):13595-13603. PMID:32940455
- 14. Yina Liao, Yao Gao, et al. "Melatonin synergizes BRAF-targeting agent dabrafenib for the treatment of anaplastic thyroid cancer by inhibiting AKT/hTERT signalling." J Cell Mol Med. 2020 Sep 15. PMID:32935463
- 15. Enkhmurun Chibaatar, Kai Le, et al. "Melatonin Ameliorates Lipopolysaccharide-Induced Microglial Inflammation via Triggering SIRT1/HMGB1 Signaling Axis." J Mol Neurosci. 2020 Sep 10. PMID:32910356
- 16. Xiaolong Tang, Guorui Sun, et al. "Circular noncoding RNA circMBOAT2 is a novel tumor marker and regulates proliferation/migration by sponging miR-519d-3p in colorectal cancer." Cell Death Dis. 2020 Aug 14;11(8):625. PMID:32796815
- 17. Jinghui Liang, Haixia Li, et al. "Mex3a interacts with LAMA2 to promote lung adenocarcinoma metastasis via PI3K/AKT pathway." Cell Death & Disease volume 11, Article number: 614 (2020);13 August 2020. PMID:32792503
- 18. Mengyao Liu, Manfei Fu, et al. "Paclitaxel and quercetin co-loaded functional mesoporous silica nanoparticles overcoming multidrug resistance in breast cancer." Colloids Surf B Biointerfaces. 2020 Dec;196:111284. PMID:32771817
- 19. Jing Yang, Bo Li, et al. "Exosomal miR-638 Inhibits Hepatocellular Carcinoma Progression by Targeting SP1." 7 July 2020 Volume 2020:13 Pages 6709—6720. PMID:32764961
- 20. Yang X, Liu H, et al. "MiR-96 promotes apoptosis of nucleus pulpous cells by targeting FRS2." Hum Cell. 2020;10.1007/s13577-020-00389-9. PMID:32578051
- 21. Mo J, Zhao Y, et al. "Circ-APBB1IP as a Prognostic Biomarker Promotes Clear Cell Renal Cell Carcinoma Progression Through The ERK1/2 Signaling Pathway." Int J Med Sci. 2020;17(9):1177-1186. PMID:32547313
- 22. Li RX, Han R, et al. "Synthesis and Biological Evaluation of Diversified Hamigeran B Analogs as Neuroinflammatory Inhibitors and Neurite Outgrowth Stimulators." Mar Drugs. 2020;18(6):306. PMID:32545418
- 23. Liu L, Wei Z, et al. "Giardia duodenalis induces extrinsic pathway of apoptosis in intestinal epithelial cells through activation of TNFR1 and K63 de-ubiquitination of RIP1 in vitro." Microb Pathog. 2020;149:104315. PMID:32525021
- 24. Gao M, Wan X, et al. "Kidney injury induced by elevated histones in community-acquired pneumonia." Mol Cell Biochem. 2020;471(1-2):155-163. PMID:32519229
- 25. Cheng Y, Chen T, et al. "Pituitary miRNAs target GHRHR splice variants to regulate GH synthesis by mediating different intracellular signalling pathways." RNA Biol. 2020;1-13. PMID:32508238
- 26. Yang D, Sun Y, et al. "REV7 is required for processing AID initiated DNA lesions in activated B cells." Nat Commun. 2020;11(1):2812. PMID:32499490
- 27. Krotova K, Aslanidi G. "Modifiers of AAV-mediated gene expression in implication for serotype-universal neutralizing antibody assay." Hum Gene Ther. 2020;10.1089/hum.2020.074. PMID:32495655
- 28. Shen N, Wang P, et al. "Nonreceptor protein tyrosine phosphatases (NRPTPs) gene family associates with the risk of hepatocellular carcinoma in a Chinese hepatitis B virus-related subjects." Mol Carcinog. 2020;59(8):980-988. PMID:32484301
- 29. Wang W, Bai J, et al. "Protective Effects of Punicalagin on Osteoporosis by Inhibiting Osteoclastogenesis and Inflammation via the NF-κB and MAPK Pathways." Front Pharmacol. 2020;11:696. PMID:32477149
- 30. Jingyuan Cao, Bin Wang, et al. "Three-dimensional culture of MSCs produces exosomes with improved yield and enhanced therapeutic efficacy for cisplatin-induced acute kidney injury." Stem Cell Res Ther. 2020 May 27;11(1):206. PMID:32460853
- 31. Tan J, Xu W, et al. "Inhibition of Aurora Kinase A by Alisertib Reduces Cell Proliferation and Induces Apoptosis and Autophagy in HuH-6 Human Hepatoblastoma Cells." Onco Targets Ther. 2020;13:3953-3963. PMID:32440158
- 32. Niu X, Lin X, et al. "Long-wave Ultraviolet Ray Promotes Inflammation in Keloid-derived Fibroblasts by Activating P38-NFκB1 Signaling Pathway." J Burn Care Res. 2020;iraa075. PMID:32433772
- 33. Kong P, Yu KN, et al. "Micheliolide Enhances Radiosensitivities of p53-Deficient Non-Small-Cell Lung Cancer via Promoting HIF-1α Degradation." Int J Mol Sci. 2020;21(9):3392. Published 2020 May 11. PMID:32403326
- 34. Zhao F, Bai R, et al. "Meconopsis horridula Hook. f. & Thomson extract and its alkaloid oleracein E exert cardioprotective effects against acute myocardial ischaemic injury in mice." J Ethnopharmacol. 2020;258:112893. PMID:32387233
- 35. Duan M, Hu F, et al. "Silencing KPNA2 inhibits IL-6-induced breast cancer exacerbation by blocking NF-κB signaling and c-Myc nuclear translocation in vitro." Life Sci. 2020;253:117736. PMID:32360571
- 36. Cai H, Lin L, et al. "Folic acid rescues corticosteroid-induced vertebral malformations in chick embryos through targeting TGF-β signaling." J Cell Physiol. 2020;10.1002/jcp.29707. PMID:32324263
- 37. Zuo Y, Shen W, et al. "Circular RNA Circ-ZNF609 Promotes Lung Adenocarcinoma Proliferation by Modulating miR-1224-3p/ETV1 Signaling." Cancer Manag Res. 2020;12:2471-2479. PMID:32308483
- 38. Zhang Y, Shi J, et al. "H2A Monoubiquitination Links Glucose Availability to Epigenetic Regulation of the Endoplasmic Reticulum Stress Response and Cancer Cell Death." Cancer Res. 2020;80(11):2243-2256. PMID:32273282
- 39. Jiang R, Gao Q, et al. "Elk-1 transcriptionally regulates ZC3H4 expression to promote silica-induced epithelial-mesenchymal transition." Lab Invest. 2020;100(7):959-973. PMID:32218530
- 40. Yang C, Zhu S, et al. "FBP1 binds to the bromodomain of BRD4 to inhibit pancreatic cancer progression." Am J Cancer Res. 2020;10(2):523-535. PMID:32195024
- 41. Jin Y, Zhang J, et al. "Expression profiles of miRNAs in giant cell tumor of bone showed miR-187-5p and miR-1323 can regulate biological functions through inhibiting FRS2." Cancer Med. 2020;9(9):3163-3173. PMID:32154662
- 42. Jin YH, Zhang J, et al. "Functions of Exogenous RUNX2 in Giant Cell Tumor of Bone In Vitro." Orthop Surg. 2020;12(2):668-678. PMID:32154660
- 43. Liu F, Shang YX. "Sirtuin 6 attenuates epithelial-mesenchymal transition by suppressing the TGF-β1/Smad3 pathway and c-Jun in asthma models." Int Immunopharmacol. 2020;82:106333. PMID:32143002
- 44. Zhang R, Zhang W, et al. "Long intergenic non-coding RNA 1939 eliminates proliferation and migration of human renal cell carcinoma (RCC) cells by down-regulation of miR-154." Artif Cells Nanomed Biotechnol. 2020;48(1):695-702. PMID:32138544
- 45. Zheng Y, Yang X, et al. "HDAC6,modulated by miR-206, promotes endometrial cancer progression through the PTEN/AKT/mTOR pathway." Sci Rep. 2020 Feb 27;10(1):3576. PMID:32107418
- 46. Cheng Y, Chen T, et al. "miR-709 inhibits GHRP6 induced GH synthesis by targeting PRKCA in pituitary." Mol Cell Endocrinol. 2020 Apr 15;506:110763. PMID:32084499
- 47. Rao SPS, Lakshminarayana SB, Jet al. "Anti-Trypanosomal Proteasome Inhibitors Cure Hemolymphatic and Meningoencephalic Murine Infection Models of African Trypanosomiasis." Trop Med Infect Dis. 2020 Feb 17;5(1). pii: E28. PMID:32079320
- 48. Zhong Z, Yao X, et al. "Protocatechuic aldehyde mitigates hydrogen peroxide-triggered PC12 cell damage by down-regulating MEG3." Artif Cells Nanomed Biotechnol. 2020;48(1):602–609. PMID:32064936
- 49. Wang Y, Shi S, et al. "MicroRNA-206 upregulation relieves circTCF25-induced osteosarcoma cell proliferation and migration." J Cell Physiol. 2020 Feb 13. PMID:32052450
- 50. Xie Y, Chen H, et al. "Inhibiting Necroptosis of Spermatogonial Stem Cell as a Novel Strategy for Male Fertility Preservation." Stem Cells Dev. 2020 Apr 15;29(8):475-487. PMID:32024413
- 51. He J, Huang Z, et al. "Circular RNA MAPK4 (circ-MAPK4) inhibits cell apoptosis via MAPK signaling pathway by sponging miR-125a-3p in gliomas." Mol Cancer. 2020 Jan 28;19(1):17. PMID:31992303
- 52. Zhang G, Zhai N, et al. "Alkannin represses growth of pancreatic cancer cells based on the down regulation of miR-199a." Biofactors. 2020 Jan 22. PMID:31967380
- 53. Zeng W, Zhang Y, et al. "Lipopolysaccharide enhances human herpesvirus 1 replication and IL-6 release in epithelial cells." Microb Pathog. 2020 Mar;140:103961. PMID:31904451
- 54. Zhang Y, Yan T, et al. "Rutaecarpine inhibits KEAP1-NRF2 interaction to activate NRF2 and ameliorate dextran sulfate sodium-induced colitis." Free Radic Biol Med. 2020 Feb 20;148:33-41. PMID:31874248
- 55. Weiguo Wang, Sailing Zhu, et al. "Antibacterial and thermomechanical properties of experimental dental resins containing quaternary ammonium monomers with two or four methacrylate groups." RSC Adv., 2019, 9,40681
- 56. Lv Q, Zhang P, et al. "Quercetin, a pneumolysin inhibitor, protects mice against Streptococcus pneumoniae infection." Microb Pathog. 2020 Mar;140:103934. PMID:31862394
- 57. Dong Y, Yan X, et al. "Notoginsenoside R1 suppresses miR-301a via NF-κB pathway in lipopolysaccharide-treated ATDC5 cells." Exp Mol Pathol. 2020 Feb;112:104355. PMID:31837326
- 58. Xing F, Li L, et al. "Surface mineralized biphasic calcium phosphate ceramics loaded with urine-derived stem cells are effective in bone regeneration." J Orthop Surg Res. 2019 Dec 9;14(1):419. PMID:31818319
- 59. Li Z, He Y, et al. "A fast-dissolving microneedle array loaded with chitosan nanoparticles to evoke systemic immune responses in mice." J Mater Chem B. 2020 Jan 14;8(2):216-225. PMID:31803892
- 60. Sun Q, Xiong K, et al. "Inhibiting Fungal Echinocandin Resistance by Small-Molecule Disruption of Geranylgeranyltransferase Type I Activity." Antimicrob Agents Chemother. 2020 Jan 27;64(2). pii: e02046-19. PMID:31791942
- 61. Du X, Li Q, et al. "Integrated Analysis of miRNA-mRNA Interaction Network in Porcine Granulosa Cells Undergoing Oxidative Stress." Oxid Med Cell Longev. 2019 Nov 4;2019:1041583. PMID:31781320
- 62. He S, Chen M, et al. "Triptolide inhibits PDGF-induced proliferation of ASMCs through G0/G1 cell cycle arrest and suppression of the AKT/NF-κB/cyclinD1 signaling pathway." Eur J Pharmacol. 2020 Jan 15;867:172811. PMID:31756335
- 63. Li H, Lou B, et al. "Ganoderic Acid A exerts the cytoprotection against hypoxia-triggered impairment in PC12 cells via elevating microRNA-153." Phytother Res. 2020 Mar;34(3):640-648. PMID:31742778
- 64. Liu X, Shortt C, et al. "Extracellular Vesicles Released From Articular Chondrocytes Play a Major Role in Cell-Cell Communication." J Orthop Res. 2020 Apr;38(4):731-739. PMID:31736104
- 65. Sun J, Wang X, et al. "circAtp9b knockdown alleviates LPS-caused inflammation provided that microRNA-27a is upregulated." Int Immunopharmacol. 2020 Jan;78:105925. PMID:31735661
- 66. Jin X, Fang R, et al. "PES1 promotes BET inhibitors resistance and cells proliferation through increasing c-Myc expression in pancreatic cancer." J Exp Clin Cancer Res. 2019 Nov 12;38(1):463. PMID:31718704
- 67. Zhan Q, Ma X, et al. "PEAR1 suppresses the proliferation of pulmonary microvascular endothelial cells via PI3K/AKT pathway in ALI model." Microvasc Res. 2020 Mar;128:103941. PMID:31678362
- 68. WeiLuo, LeiAi, et al. "High glucose inhibits myogenesis and induces insulin resistance by down-regulating AKT signaling." Biomedicine & Pharmacotherapy. Volume 120, December 2019, 109498.
- 69. Huang T, Fu Y, et al. "miR-96 acts as a tumor suppressor via targeting the BCR-ABL1 oncogene in chronic myeloid leukemia blastic transformation." Biomed Pharmacother. 2019 Nov;119:109413. PMID:31518872
- 70. Han Y, Kang C, et al. "Long non-coding RNA Mirt2 prevents TNF-α-triggered inflammation via the repression of microRNA-101." Int Immunopharmacol. 2019 Nov;76:105878. PMID:31513985
- 71. Wu M, Chen W, et al. "Rotenone protects against β-cell apoptosis and attenuates type 1 diabetes mellitus." Apoptosis. 2019 Dec;24(11-12):879-891. PMID:31485878
- 72. Li ZL, Lv LL, et al. "The profibrotic effects of MK-8617 on tubulointerstitial fibrosis mediated by the KLF5 regulating pathway." FASEB J. 2019 Nov;33(11):12630-12643. PMID:31451021
- 73. Hou S, Shan M, et al. "PCDHGB7 Increases Chemosensitivity to Carboplatin by Inhibiting HSPA9 via Inducing Apoptosis in Breast Cancer." Dis Markers. 2019 Jul 8;2019:6131548. PMID:31379979
- 74. Le K, Chibaatar Daliv E, et al. "SIRT1-regulated HMGB1 release is partially involved in TLR4 signal transduction: A possible anti-neuroinflammatory mechanism of resveratrol in neonatal hypoxic-ischemic brain injury." Int Immunopharmacol. 2019 Oct;75:105779. PMID:31362164
- 75. Liu L, Chen Y, et al. "Silencing of KCNA1 suppresses the cervical cancer development via mitochondria damage." Channels (Austin). 2019 Dec;13(1):321-330. PMID:31354026
- 76. Li Y, Song D, et al. "Diazepam inhibited lipopolysaccharide (LPS)-induced pyroptotic cell death and alleviated pulmonary fibrosis in mice by specifically activating GABA(A) receptor α4-subunit." Biomed Pharmacother. 2019 Oct;118:109239. PMID:31351431
- 77. Zhao J, Cao J, et al. "Dehydroepiandrosterone alleviates E. Coli O157:H7-induced inflammation by preventing the activation of p38 MAPK and NF-κB pathways in mice peritoneal macrophages." Mol Immunol. 2019 Oct;114:114-122. PMID:31351412
- 78. Li T, Wei Z, et al. "Withanolides, Extracted from Datura Metel L. Inhibit Keratinocyte Proliferation and Imiquimod-Induced Psoriasis-Like Dermatitis via the STAT3/P38/ERK1/2 Pathway." Molecules. 2019 Jul 17;24(14). pii: E2596. PMID:31319488
- 79. Guo G, Gong L, et al. "Quercetin supports cell viability and inhibits apoptosis in cardiocytes by down-regulating miR-199a." Artif Cells Nanomed Biotechnol. 2019 Dec;47(1):2909-2916. PMID:31307244
- 80. Wu M, Li S, et al. "Mitochondrial activity contributes to impaired renal metabolic homeostasis and renal pathology in STZ-induced diabetic mice." Am J Physiol Renal Physiol. 2019 Sep 1;317(3):F593-F605. PMID:31268353
- 81. Zhang B, Chen M, et al. "A regulatory circuit of circ-MTO1/miR-17/QKI-5 inhibits the proliferation of lung adenocarcinoma." Cancer Biol Ther. 2019;20(8):1127-1135. PMID:30975029
- 82. Song S, Jiang J, et al. "Structural optimization on a virtual screening hit of smoothened receptor." Eur J Med Chem. 2019 Jun 15;172:1-15. PMID:30939349
- 83. Guo X, Wang Z, et al. "Upregulated KPNA2 promotes hepatocellular carcinoma progression and indicates prognostic significance across human cancer types." Acta Biochim Biophys Sin (Shanghai). 2019 Mar 1;51(3):285-292. PMID:30883648
- 84. Lv X, Song J, et al. "Core fucosylation of copper transporter 1 plays a crucial role in cisplatin-resistance of epithelial ovarian cancer by regulating drug uptake." Mol Carcinog. 2019 Jan 5. PMID:30614075
Related Biological Data
Related Biological Data
Related Biological Data
Related Biological Data
Related Biological Data
Related Biological Data
Related Biological Data
Related Biological Data
| 100 tests | 1 mL x 1 |
| 500 tests | 5 mL x 1 |
| 1000 tests | 5 mL x 2 |
| 3000 tests | 5 mL x 6 |
| 10000 tests | 5 mL x 20 |
| CCK-8 is stable exceeding one year at 0-5°C with protection from light. For longer storage, store it at -20°C. Repeated thawing and freezing will cause an increase in the background, which will interfere with your assay. Please store the kit at 0-5°C for frequent use. | |
Our product Cell Counting Kit-8 (CCK-8) provide a more convenient and sensitive method for the research of cell number determination and cell proliferationtotoxicity assay than previous ways. The kit utilizes a highly water-soluble tetrazolium salt, WST-8, which produces a water-soluble formazan dye upon reduction in the presence of an electron mediator. The amount of the formazan generated by dehydrogenases is directly in proportion to the numbers of living cells. The detection sensitivity by CCK-8 is higher than other tetrazolium salts such as MTT, XTT, MTS or WST-1.
More Sensitive than MTT, MTS, or WST-1
No Toxicity to Cell
Simpler Steps, No organic solvents required
Stable One Bottle Solution, ready-to-use
Advantages
Store at 4°C.