Live-Dead Cell Staining Kit I (Calcein AM/PI)
- 1. Jiamin Qin, Guojuan Fan, et al. "Dynamic Covalent Bond-Based Nanoassembly of Curcumin to Enhance the Selective Photothermal Therapy for Tumor Treatment." International Journal of Nanomedicine 02 Apr 2025
- 2. Wenhui Yang, Yilu Li, et al. "Mesenchymal stem cells overexpressing neuropeptide S promote the recovery of rats with spinal cord injury by activating the PI3K/AKT/GSK3β signaling pathway." Stem Cell Res Ther.2025 Feb 28;16(1):100. PMID: 40022159
- 3. Mei Zhang, Qiuxia Tan, et al. "Carrier-Free Cisplatin–Dactolisib Nanoparticles for Enhanced Synergistic Antitumor Efficacy." ACS Biomater Sci Eng. 2025 Mar 10;11(3):1456-1471. PMID: 39992316
- 4. Yizuo Song, Xin Shou, et al. "Cell Membranes from Tumor‐Tropic MSCs Screened by a Microfluidic Chip for Drug Nanoparticles Encapsulation and Cancer Targeted Therapy." Adv Healthc Mater. 2023 Jul;12(17):e2202904. PMID: 36815538
Live-Dead Cell Staining Kit I (Calcein AM/PI) is used for simultaneous fluorescence staining of live and dead mammalian cells. This kit contains two probes, Calcein AM and propidium iodide (PI). Calcein AM is a live-cell probe, which is highly lipophilic and cell permeable. Calcein AM itself is no-fluorescent, but it is hydrolyzed into green-fluorescent Calcein by esterase in the viable cells. On the other hand, PI is a dead cell probe that can only pass through damaged cell membranes and stain the nucleus red. The fluorescence intensity of these two probes can reflect cell viability and toxicity.
The kit is sensitive, quick and easy to use. However, Calcein AM does not penetrate the cell walls of bacteria or fungi, so this kit is not suited for bacteria or fungi.
Figure 1: The effect of this kit for the detection of live/dead cells
| Components | K2247-100T | K2247-500T | K2247-2500T |
| Calcein AM (1000x) | 10 μL | 50 μL | 250 μL |
| PI (1000x) | 10 μL | 50 μL | 250 μL |
| Staining Buffer | 10 mL | 50 mL | 250 mL |
Store the kit at -20°C, stable for 1 year. The Calcein AM (1000x) and PI (1000x) should be stored away from light and moisture, avoiding repeated freeze/thaw cycles. | |||