APExBIO offers milligram to gram-scale high-purity RNA and in vitro transcription platform, including
independently
manufactured modified nucleotides, transcriptase, and tailing enzyme. We also provide a wide range of products for
mRNA research, such as reagents for synthesis, extraction, purification, analysis and detection. Our unique mRNA
purification technology can be used to quickly screen a variety of mRNA modifications to adapt to different target
cells and immunogenicity screening.
APExBIO provides one-stop RNA synthesis services, including linear, circular, and self-amplifying RNA. Our
integrated workflow covers gene synthesis, plasmid preparation, RNA synthesis, and subsequent lipid nanoparticle
(LNP) encapsulation. We deliver high-purity, superior-quality RNA with high protein expression efficiency. From
microgram to gram scales, our flexible specifications can meet your diverse research needs.
Features
Linear RNA Synthesis
Capping and Modified Nucleotides
Sequence Optimization
Microgram to Gram Scale
Circular RNA Synthesis
Capping and Modified Nucleotides
Sequence Optimization
Microgram to Gram Scale
Self-Amplifying mRNA Synthesis
Optimized Vector and Replicase
Capping and Modified Nucleotides
Less for Higher Expression Level
LNP Encapsulation
High Encapsulation Efficiency
Compatible with Different Vectors
Dry Ice Shipping
Quality Control
Type
Specification
Standard QC
Extra QC
RNA Synthesis
Appearance
Agarose gel electrophoresis
Capillary electrophoresis
mRNA concentration
A260/280
Buffer
Capping efficiency
Poly A length
RNA sequencing
Endotoxin level
√
√
√
√
√
√
√
√
√
√
LNP Encapsulation
Appearance
Concentration
Capillary electrophoresis
A260/280
Encapsulation
Particle size
PDI
Zeta potential
√
√
√
√
√
√
√
√
Catalog mRNA - Premade mRNA/circRNA/saRNA
At APExBIO, we offer a comprehensive catalog of premade RNA products, including messenger RNA (mRNA), circular RNA
(circRNA), and self-amplifying RNA (saRNA), which are incoperated with EZ Cap (Cap1) or ARCA (Cap0), polyA Tail
and Modified Nucleotides（e.g. pseudo-UTP, 5-moUTP).
These ready-to-use RNAs are meticulously synthesized, purified, and quality-controlled to ensure high integrity,
potency, and performance in a variety of applications—from transfection assays and protein expression studies to
vaccine research and gene function analysis.
Comparison
Type
Linear mRNA
Circular RNA
Self-amplifying RNA
Structure
Linear. 5' and 3' ends.
Closed Circle.
Linear. 5' and 3' ends.
Function of Cap1/cap0
Protect from Degradation. Facilitate
Nuclear Export and Splicing.
No need of Cap
Protect from Degradation. Facilitate
Nuclear Export and Splicing.
Function of polyA Tail
Enhance RNA Stability.Facilitate
Translation
No need of polyA tail
Enhance RNA Stability. Facilitate
Translation
Function of Modified Nucleotides
Reduce lmmunogenicity.
Facilitate Translation
No need of Modified Nucleotides
Reduce lmmunogenicity.
Facilitate Translation
Ribosome Recruitment
Need 5'Cap for Ribosome Recruit-
ment. and Translation Initiation
Need Internal Ribosome Entry Site
(IRES) for Translation Initiation
Need 5'Cap for Ribosome Recruit-
ment. and Translation Initiation
APExBIO offers gram-scale high-purity mRNA and in vitro transcription platform, including independently
manufactured modified nucleotides, transcriptase, and tailing enzyme. We also provide a wide range of products for
mRNA research, such as reagents for synthesis, extraction, purification, analysis and detection. Our unique mRNA
purification technology can be used to quickly screen a variety of mRNA modifications to adapt to different target
cells and immunogenicity screening.
During in vitro transcription, normal nucleotides can be replaced by synthetic nucleotide analogues such as
pseudo-UTP and 5mCTP, the resulting mRNA is called nucleotide-modified. For example, 5-methoxyuridine (5mo-UTP) is
an effective modification to eliminate innate immune stimulation, enhance translation efficiency and reduce
interferon induction. APExBIO provide a wide range of modified nucleotides including the ones labeled with
fluorescence or biotin can be used directly for the in vitro synthesis of labeled mRNA, facilitating subsequent
qualitative and quantitative analyses.
APExBIO produces various GMP-grade products as raw materials for mRNA manufacturing process. These products meet
industry specifications and are manufactured in the quality infrastructures. Our qualified equipment, utilities,
QC test methods and regulated manufacturing processes can ensure high consistency from batch-to-batch.
