EZ Cap™ Cy5 EGFP mRNA (5-moUTP)

EZ Cap™ Cy5 EGFP mRNA (5-moUTP) is a superior dual-fluorescence reporter tool engineered for advanced gene delivery research and real-time analysis. This product integrates the tracking capability of Cy5 with the functional reporting of EGFP protein expression, providing a ready-to-use tool for validating and optimizing delivery systems. EZ Cap™ Cy5 EGFP mRNA (5-moUTP) is widely used in macrophage-targeted therapy development, nanoparticle validation, and quantitative transfection studies.

Key features:

Fluorescent Labeling: The mRNA is covalently conjugated with Cy5 dye, enabling direct visualization of mRNA delivery, cellular uptake, and intracellular trafficking using fluorescence microscopy or flow cytometry, without the need for secondary detection steps.

Cap1 Structure: The mRNA incorporates a Cap1 analog at the 5' end, ensuring high translation initiation efficiency, improved stability, and reduced innate immune recognition, leading to stronger and more sustained luciferase expression.

Modified Nucleotides: It contains 5-methoxyuridine (5-moU) modified nucleotides, which decreases immunogenicity, enhances mRNA stability, and increases translational efficiency, resulting in more reliable and reproducible protein yield.