EZ Cap™ Cas9 mRNA (5-moUTP)
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
The Cas9/guide RNA (Cas9/gRNA) system is commonly used for genome editing. Upon delivery of these components to the nucleus of a cell, the RNA guide sequence targets the site of interest and the Cas9 protein is employed to perform the DNA cleavage. 5moUTP-modified Cas9 mRNA is capped using Cap 1, a novel co-transcriptional capping method, which yields natural Cap 1 with high capping efficiency. 5moUTP modification reduces innate immune responses and increases Cas9 activity.
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