In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Cy5-UTP (Cyanine 5-UTP) can replace UTP as a substrate for T7 RNA polymerase and generate labeled probes through in vitro transcription. The resulting assembled mRNA will emit orange fluorescence with the involvement of Cy5-UTPs. In addition, Cy5-labeled mRNA is readily visible under ultraviolet light without further staining after gel electrophoresis.
Probes generated by these methods are suitable for various application, such as FISH, multicolor fluorescence analysis, specifically dual-color expression arrays in conjunction with Cy5-UTP.
Excitation/Emission (nm): 650/665 nm
|Storage||Store at -20°C|
|M.Wt||1178.01 g/mol(free acid)|
|Synonyms||5-(3-Aminoallyl)-uridine-5'-triphosphate;labeled with Cy5, Triethylammonium salt, Aminoallyl-UTP-Cy5, Cyanine 5-AA-UTP, Cyanine 5-Aminoallyluridine-5'-Triphosphate, Cyanine 5-UTP|
|Solubility||Soluble in H2O|
|Canonical SMILES||O=S(C1=CC2=C(N(CCCCCC(NC/C=C/C3=CN([[email protected]]4[[email protected]](O)[[email protected]](O)[[email protected]@H](COP(OP(O)(OP(O)(O)=O)=O)(O)=O)O4)C(NC3=O)=O)=O)/C(C2(C)C)=C/C=C/C=C/C(C5(C)C)=[N+](CC)C6=C5C=C(S([O-])(=O)=O)C=C6)C=C1)(O)=O|
|Shipping Condition||Evaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.|
|General tips||For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.|