EZ Cap™ Cy5 Firefly Luciferase mRNA (5-moUTP)

EZ Cap^™ Cy5 Firefly Luciferase mRNA (5-moUTP) is a high-performance, dual-reporting mRNA product designed for advanced gene expression and intracellular tracking studies. It combines robust protein expression with sensitive fluorescence detection in a single construct. This ready-to-use mRNA is ideal for real-time tracking of mRNA delivery, optimizing transfection protocols, and dual-modality studies combining bioluminescence imaging with fluorescence-based localization. It serves as a valuable tool in mRNA vaccine development, gene therapy research, and intracellular trafficking assays.

Key features:

Fluorescent Labeling: The mRNA is covalently conjugated with Cy5 dye, enabling direct visualization of mRNA delivery, cellular uptake, and intracellular trafficking using fluorescence microscopy or flow cytometry, without the need for secondary detection steps.

Cap1 Structure: The mRNA incorporates a Cap1 analog at the 5' end, ensuring high translation initiation efficiency, improved stability, and reduced innate immune recognition, leading to stronger and more sustained luciferase expression.

Modified Nucleotides: It contains 5-methoxyuridine (5-moU) modified nucleotides, which decreases immunogenicity, enhances mRNA stability, and increases translational efficiency, resulting in more reliable and reproducible protein yield.