Fluorescent labeled mRNAs

Fluorescent labeled mRNAs are essential tools in molecular and cellular biology research. They are generated by covalently attaching a fluorophore (such as Cy3, Cy5, etc.) to the mRNA molecule, primarily used for the visualization, tracking, and quantitative analysis of mRNA. After transfecting the fluorescent labeled mRNA into living cells, researchers can observe in real-time how mRNA is transported from the nucleus to the cytoplasm, as well as its diffusion and directed transport within the cytoplasm. In the development and optimization of delivery systems during the development of mRNA vaccines, gene therapy drugs, or novel transfection reagents, using fluorescent labeled mRNA allows for the intuitive assessment of transfection/delivery efficiency, tracking of delivery carriers, and observation of the distribution of lipid nanoparticles (LNPs) or other carriers within cells, as well as the location and timing of mRNA release, thereby optimizing delivery parameters.