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Molecular Biology Reagents

Explore APExBIO reagents for essential DNA and RNA workflows—from sample preparation and cloning to amplification, reverse transcription and nucleic-acid analysis. This selection brings together widely used kits, enzymes and nucleotides, with representative experimental data where available.

Recombination & Cloning

From preparing plasmid DNA to assembling fragments and checking the final construct, cloning involves several distinct steps. These products cover DNA cleanup, plasmid preparation, assembly and gel visualization without filling the list with multiple versions of the same kit.

Catalog No.Featured ProductApplication
A8743Safe DNA Gel StainSensitive DNA/RNA gel staining
K1811DNA Clean BeadsDNA fragment purification and size selection
A5001ApexPrep DNA Plasmid Miniprep KitPlasmid DNA purification
K1054Hyper Assembly Cloning KitDirectional cloning of DNA fragments
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PCR

Choose a ready-to-use mix for routine PCR, a high-fidelity polymerase for demanding templates, or an application-specific kit for genotyping and contamination screening. The selection represents different PCR tasks rather than several closely related formulations of one master mix.

Catalog No.Featured ProductApplication
K10342X Taq PCR Master Mix (with dye)Routine PCR with simplified reaction setup
K2821PCR Mycoplasma Detection KitPCR-based mycoplasma contamination screening
K1025Direct Mouse Genotyping KitDirect PCR from mouse tissue
K1031HyperPFU™ High-Fidelity DNA PolymeraseHigh-fidelity PCR amplification of long or GC-rich DNA fragments
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qPCR

Real-time PCR reagents support fluorescence-based monitoring of target amplification. This group includes standard and fast Green-dye mixes alongside a probe-based formulation, allowing researchers to choose a format that suits their assay design and instrument workflow.

Catalog No.Featured ProductApplication
K1070HotStart™ 2X Green qPCR Master MixGreen dye-based real-time PCR
K1170HotStart™ Universal 2X Green qPCR Master MixUniversal green dye-based qPCR
K1541HotStart™ 2X Probe qPCR Master MixProbe-based real-time PCR
K1172HotStart™ Universal 2X FAST Green qPCR Master MixFast Green-dye qPCR workflows
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Reverse Transcription

Reverse transcription converts RNA into cDNA for subsequent PCR and qPCR analysis. Select a standalone reverse transcriptase when you need to configure the reaction, or a first-strand synthesis kit or premix when a simpler setup better fits the workflow.

Catalog No.Featured ProductApplication
K1072HyperScript™ First-Strand cDNA Synthesis KitFirst-strand cDNA synthesis from RNA
K1074HyperScript™ RT SuperMix for qPCRcDNA synthesis for downstream qPCR
K1073HyperScript™ First-Strand cDNA Synthesis SuperMixPremixed first-strand cDNA synthesis
K1071HyperScript™ Reverse TranscriptaseStandalone enzyme for cDNA synthesis
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NGS Library Preparation

Library preparation brings together RNA enrichment, fragmentation, adapter-related processing and complete library construction. The four products below follow APExBIO's NGS Library Preparation category, making it easier to find reagents for individual steps or a premixed workflow.

Catalog No.Featured ProductApplication
K18155′ DNA Adenylation KitPreparation of 5′-adenylated DNA adapters
K18142× FragPrime Buffer for RNA Library PrepRNA fragmentation and first-strand cDNA priming
K1813mRNA Magnetic Purification Kit for RNA Library PrepmRNA enrichment from total RNA
K1810RNA Library Prep Kit (Premixed Version)RNA library construction
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Enzymes

Enzymes support DNA and RNA preparation, digestion, end processing and ligation throughout molecular biology workflows. This selection spans protease, nuclease, exonuclease and ligase activities, so researchers can identify the function needed for a particular step.

Catalog No.Featured ProductApplication
K1037Proteinase KProtein digestion during sample preparation
K1093RNase H (RNase-free)RNA-strand cleavage in DNA–RNA hybrids
K1088DNase I (RNase-free)Removal of DNA from RNA preparations
K1094T5 Exonuclease (RNase-free)5′-to-3′ DNA end processing
K1100T4 DNA Ligase (RNase-free)Joining DNA fragments and repairing nicks
K1104Dpn IDigestion of methylated DNA
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Nucleotides

Standard and modified nucleotides provide building blocks for DNA synthesis, labeling and method development. The products below represent a dNTP mixture alongside chain-terminating, oxidized, methylated, biotinylated and fluorescently labeled nucleotides.

Catalog No.Featured ProductApplication
B8136ddATPChain-terminating nucleotide analogue
B80998-Oxo-dGTPOxidized guanine nucleotide analogue
B8151Biotin-16-dCTPBiotin labeling of DNA
B8093N6-Methyl-dATPMethylated nucleotide analogue
K104225 mM dNTP MixtureEquimolar dNTP mixture for DNA synthesis
B8202Cy3-dUTPFluorescent labeling of DNA or cDNA
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FAQ

  • Can Safe DNA Gel Stain (A8743) be viewed with blue light?

    Yes. It can be excited with blue light as well as UV; APExBIO recommends blue light to reduce DNA damage during gel imaging. Product FAQ ↗

  • Can Safe DNA Gel Stain (A8743) be added during gel preparation?

    Yes. The concentrate can be added to the gel at 1:10,000, or used for post-electrophoresis staining at 1:3,300. Product FAQ ↗

  • Is Safe DNA Gel Stain (A8743) suitable for very short fragments?

    It is less effective for nucleic-acid fragments around 100–200 bp; check the product guidance before choosing it for small bands. Product FAQ ↗

  • Must tissue look fully digested before PCR with Direct Mouse Genotyping Kit (K1025)?

    Not necessarily. For most samples, 15 minutes with Proteinase K at 56°C is sufficient even if some tissue remains visible. Product FAQ ↗

  • Can PCR products from Direct Mouse Genotyping Kit (K1025) be loaded directly onto a gel?

    Yes. The included PCR master mix contains loading dye for direct gel loading after amplification. Product FAQ ↗

  • What can cause nonspecific bands with Direct Mouse Genotyping Kit (K1025)?

    Possible causes include primer design, too much DNA template, a low annealing temperature or reaction-setup conditions. Product FAQ ↗


Product Citations

Need help choosing a kit? View the complete Molecular Biology Kits portfolio or contact APExBIO technical support.