Molecular Biology Reagents
Explore APExBIO reagents for essential DNA and RNA workflows—from sample preparation and cloning to amplification, reverse transcription and nucleic-acid analysis. This selection brings together widely used kits, enzymes and nucleotides, with representative experimental data where available.
Recombination & Cloning
From preparing plasmid DNA to assembling fragments and checking the final construct, cloning involves several distinct steps. These products cover DNA cleanup, plasmid preparation, assembly and gel visualization without filling the list with multiple versions of the same kit.
| Catalog No. | Featured Product | Application |
|---|---|---|
| A8743 | Safe DNA Gel Stain | Sensitive DNA/RNA gel staining |
| K1811 | DNA Clean Beads | DNA fragment purification and size selection |
| A5001 | ApexPrep DNA Plasmid Miniprep Kit | Plasmid DNA purification |
| K1054 | Hyper Assembly Cloning Kit | Directional cloning of DNA fragments |
PCR
Choose a ready-to-use mix for routine PCR, a high-fidelity polymerase for demanding templates, or an application-specific kit for genotyping and contamination screening. The selection represents different PCR tasks rather than several closely related formulations of one master mix.
| Catalog No. | Featured Product | Application |
|---|---|---|
| K1034 | 2X Taq PCR Master Mix (with dye) | Routine PCR with simplified reaction setup |
| K2821 | PCR Mycoplasma Detection Kit | PCR-based mycoplasma contamination screening |
| K1025 | Direct Mouse Genotyping Kit | Direct PCR from mouse tissue |
| K1031 | HyperPFU™ High-Fidelity DNA Polymerase | High-fidelity PCR amplification of long or GC-rich DNA fragments |
qPCR
Real-time PCR reagents support fluorescence-based monitoring of target amplification. This group includes standard and fast Green-dye mixes alongside a probe-based formulation, allowing researchers to choose a format that suits their assay design and instrument workflow.
| Catalog No. | Featured Product | Application |
|---|---|---|
| K1070 | HotStart™ 2X Green qPCR Master Mix | Green dye-based real-time PCR |
| K1170 | HotStart™ Universal 2X Green qPCR Master Mix | Universal green dye-based qPCR |
| K1541 | HotStart™ 2X Probe qPCR Master Mix | Probe-based real-time PCR |
| K1172 | HotStart™ Universal 2X FAST Green qPCR Master Mix | Fast Green-dye qPCR workflows |
Reverse Transcription
Reverse transcription converts RNA into cDNA for subsequent PCR and qPCR analysis. Select a standalone reverse transcriptase when you need to configure the reaction, or a first-strand synthesis kit or premix when a simpler setup better fits the workflow.
| Catalog No. | Featured Product | Application |
|---|---|---|
| K1072 | HyperScript™ First-Strand cDNA Synthesis Kit | First-strand cDNA synthesis from RNA |
| K1074 | HyperScript™ RT SuperMix for qPCR | cDNA synthesis for downstream qPCR |
| K1073 | HyperScript™ First-Strand cDNA Synthesis SuperMix | Premixed first-strand cDNA synthesis |
| K1071 | HyperScript™ Reverse Transcriptase | Standalone enzyme for cDNA synthesis |
NGS Library Preparation
Library preparation brings together RNA enrichment, fragmentation, adapter-related processing and complete library construction. The four products below follow APExBIO's NGS Library Preparation category, making it easier to find reagents for individual steps or a premixed workflow.
| Catalog No. | Featured Product | Application |
|---|---|---|
| K1815 | 5′ DNA Adenylation Kit | Preparation of 5′-adenylated DNA adapters |
| K1814 | 2× FragPrime Buffer for RNA Library Prep | RNA fragmentation and first-strand cDNA priming |
| K1813 | mRNA Magnetic Purification Kit for RNA Library Prep | mRNA enrichment from total RNA |
| K1810 | RNA Library Prep Kit (Premixed Version) | RNA library construction |
Enzymes
Enzymes support DNA and RNA preparation, digestion, end processing and ligation throughout molecular biology workflows. This selection spans protease, nuclease, exonuclease and ligase activities, so researchers can identify the function needed for a particular step.
| Catalog No. | Featured Product | Application |
|---|---|---|
| K1037 | Proteinase K | Protein digestion during sample preparation |
| K1093 | RNase H (RNase-free) | RNA-strand cleavage in DNA–RNA hybrids |
| K1088 | DNase I (RNase-free) | Removal of DNA from RNA preparations |
| K1094 | T5 Exonuclease (RNase-free) | 5′-to-3′ DNA end processing |
| K1100 | T4 DNA Ligase (RNase-free) | Joining DNA fragments and repairing nicks |
| K1104 | Dpn I | Digestion of methylated DNA |
Nucleotides
Standard and modified nucleotides provide building blocks for DNA synthesis, labeling and method development. The products below represent a dNTP mixture alongside chain-terminating, oxidized, methylated, biotinylated and fluorescently labeled nucleotides.
| Catalog No. | Featured Product | Application |
|---|---|---|
| B8136 | ddATP | Chain-terminating nucleotide analogue |
| B8099 | 8-Oxo-dGTP | Oxidized guanine nucleotide analogue |
| B8151 | Biotin-16-dCTP | Biotin labeling of DNA |
| B8093 | N6-Methyl-dATP | Methylated nucleotide analogue |
| K1042 | 25 mM dNTP Mixture | Equimolar dNTP mixture for DNA synthesis |
| B8202 | Cy3-dUTP | Fluorescent labeling of DNA or cDNA |
FAQ
Can Safe DNA Gel Stain (A8743) be viewed with blue light?
Yes. It can be excited with blue light as well as UV; APExBIO recommends blue light to reduce DNA damage during gel imaging. Product FAQ ↗
Can Safe DNA Gel Stain (A8743) be added during gel preparation?
Yes. The concentrate can be added to the gel at 1:10,000, or used for post-electrophoresis staining at 1:3,300. Product FAQ ↗
Is Safe DNA Gel Stain (A8743) suitable for very short fragments?
It is less effective for nucleic-acid fragments around 100–200 bp; check the product guidance before choosing it for small bands. Product FAQ ↗
Must tissue look fully digested before PCR with Direct Mouse Genotyping Kit (K1025)?
Not necessarily. For most samples, 15 minutes with Proteinase K at 56°C is sufficient even if some tissue remains visible. Product FAQ ↗
Can PCR products from Direct Mouse Genotyping Kit (K1025) be loaded directly onto a gel?
Yes. The included PCR master mix contains loading dye for direct gel loading after amplification. Product FAQ ↗
What can cause nonspecific bands with Direct Mouse Genotyping Kit (K1025)?
Possible causes include primer design, too much DNA template, a low annealing temperature or reaction-setup conditions. Product FAQ ↗

