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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
DNase I (RNase-free) is an endonuclease that digests single- or double- stranded DNA, generating dinucleotide, trinucleotide, and oligonucleotide products with 5´-phosphorylated and 3´-hydroxylated ends. The activity of DNase I (RNase-free) dependent on Ca2+ and can be activated by Mg2+ or Mn2+. In the presence of Mg2+, DNase I (RNase-free) can randomly cleaves arbitrary sites of double-stranded DNA; in the presence of Mn2+, DNase I (RNase-free) can simultaneously recognize both strands of DNA and cleaves at nearly same sites.
DNase I (RNase-free) can act on single stranded DNA, double stranded DNA, chromatin, and RNA: DNA hybrid strands. DNase I (RNase-free) is suitable for RNA extraction, in vitro transcription, and removal of DNA for RT-PCR experiments.
Store the components at -20°C.