2× FragPrime Buffer for RNA library Prep

2×FragPrime Buffer for RNA library Prep is a specialized reagent for RNA fragmentation and first-strand cDNA synthesis in RNA library preparation. Under high-temperature conditions, RNA is fragmented into short fragments through the function of Mg²⁺ ions. The fragment size can be controlled by adjusting the incubation time and temperature. The RNA used can be total RNA, purified mRNA, or RNA products after rRNA depletion. If the RNA sample is already dissolved in nuclease-free ddH₂O, this product can be selected for RNA fragmentation and subsequent first-strand cDNA synthesis reactions. This product should be used in combination with the RNA Library Prep Kit (Premixed Version) (Cat. No. K1810).