Safe DNA Gel Stain is a highly sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels. It is specifically formulated to be a less hazardous alternative to ethidium bromide (EB) that can be used with either blue-light or UV excitation.
Safe DNA Gel Stain is a better nucleic acid staining reagent for all your molecular biology research needs. It is not only environmental friendly but also good for your sample.
Safe DNA Gel Stain is supplied as 10000X concentrate in DMSO and used in the same way as ethidium bromide solution. It is also suitable for staining RNA in gels.
Cloning efficiency of DNA fragments is improved by using Safe DNA Gel Stain and blue-light, compared with using ethidium bromide and UV light exposure.
• Reduce exposure to highly mutagenic EB and harmful UV light.
Safe DNA Gel Stain is less mutagenic and safer for you to work with than ethidium bromide. You can further decrease your exposure risk by using visible blue-light with Safe stain rather than UV. This is especially valuable when performing exposure-intensive protocols like cutting bands out of gels.
• Increase sensitivity by reducing nonspecific background fluorescence.
Safe DNA Gel Stain offers excellent sensitivity in nucleic acid visualization with either UV excitation or blue-light excitation. When bound to nucleic acids, the green-fluorescent Safe stain has fluorescence excitation max at ~280 and ~502 nm, and an emission max at ~530 nm. Plus, when used with blue light, Safe DNA Gel Stain has less background fluorescence than ethidium bromide–stained gels with UV light.
• Alternative to EB for all staining applications, including RNA staining.
Safe DNA Gel Stain is supplied as a concentrate in DMSO which can be used in the same way as ethidium bromide. It can be added into an agarose gel for staining during electrophoresis at a ratio of 1: 10000 or apply it after electrophoresis at a ratio of 1: 3300. Store at room temperature in its original packaging to avoid excessive light exposure.
Note: Safe DNA Gel Stain is less efficient in visualizing low molecule weight bands (100-200 bp).