Genotyping Kit
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
The Genotyping Kit for target alleles of insects, tissues, fishes and cells provides convenience in faster preparation and superior PCR amplification. Lysis buffer and balance buffer could digest tissues or cells rapidly to release unbroken genomic DNA, and it could be used as PCR template directly without extraction. There is no need for overnight digestion, Phenol/Chloroform extraction, toilsome manual purification or the utilization of expensive DNA spin columns. By using our kit, you can substantially shorten your time for digestion. Furthermore, the 2×PCR Master Mix (With Dye) will guarantee a robust and accurate amplification consequence. This Master Mix contains dye which means it can be directly electrophoresed after the amplification without the need to add a loading buffer. In addition, our genotyping Kit mitigates the risk of sample cross-contamination occurring in the PCR reaction by conducting the DNA extraction in one single tube.
| Complete Kit | 200 rxns | 500 rxns |
| Lysis buffer | 20 mL | 50 mL |
| Balance buffer | 20 mL | 50 mL |
| 2×PCR Master Mix (With Dye) | 1 mL x 2 | 1 mL x 5 |
| Proteinase K | 200 μl | 500 μl |
1. Lysis buffer and Balance buffer should be stored at 4°C. 2. 2 x PCR Master Mix could be stored at -20°C for 2 years. 3. Store the unopened Protease K at -20 to -70 °C. Avoid repeated freeze/thaw cycles. Upon first use, it is recommended to aliquot sample into single use portions. The Proteinase K solution should be stored at -20°C and could be stored at 4°C for a short time. | ||
