RNase Inhibitor, Murine

Murine RNase inhibitor is a 50 kDa recombinant protein derived from a mouse RNase inhibitor gene expressed in E.coli. It specifically and non-covalently interacts in a 1:1 ratio with pancreatic-type RNases such as RNase A, B, and C, without affecting RNase 1, RNase T1, RNase H, S1 nuclease, or fungal RNases. This inhibitor demonstrates resistance to oxidative inactivation due to absence of oxidation-sensitive cysteines found in human-derived forms, and maintains activity under low reducing conditions (below 1 mM DTT). Typically applied at concentrations of 0.5–1 U/μL to prevent RNA degradation, it is suitable for real-time RT-PCR, cDNA synthesis, in vitro transcription, RNA enzymatic labeling, and related RNA-based molecular biology assays.

The product inhibits RNases A, B and C specifically.

Improved resistance to oxidation compared to human/porcine RNase inhibitor

Ideal for reactions when low DTT concentration is necessary (e.g., Real-time PCR)