Oligo (dT) 25 Beads
mRNA accounts for only 1%-5% of the total RNA in eukaryotic cells, making efficient purification of mRNA essential for subsequent experiments. Oligo (dT) 25 Beads are specifically designed for purification of eukaryotic mRNA. These beads are monodisperse superparamagnetic particles functionalized on their surface with covalently bound oligo (dT) sequences. By relying on complementary base pairing between the oligo (dT) sequences and the polyA tail of mRNA, these beads can quickly isolate highly purified, intact mRNA directly from total RNA or from eukaryotic cells and animal or plant tissues.
The isolated mRNA can be directly used in first-strand cDNA synthesis reactions with the bound oligo (dT) sequence serving as a primer, or it can be eluted from the magnetic beads and utilized in different downstream molecular experiments, such as RT-PCR, RPA (Ribonuclease Protection Assay), library construction, Northern Blot analysis, and next-generation sequencing.
- 1. Pu-Yang Sun, Jie Liu, et al. "Rejuvenation of peripheral immune cells attenuates Alzheimer’s disease-like pathologies and behavioral deficits in a mouse model." Sci Adv. 2024 May 31;10(22):eadl1123 PMID: 38809977
- 2. Mengjun Zhang, Zhuang Gu, et al. "SRRM2 phase separation drives assembly of nuclear speckle subcompartments." Cell Rep. 2024 Mar 26;43(3):113827. PMID: 38381607
- 3. Jiangnan Huang, Linjie Rao, et al. "Effect of crossbreeding and sex on slaughter performance and meat quality in Xingguo gray goose based on multiomics data analysis." Poult Sci. 2023 Aug;102(8):102753. PMID: 37267641
- 4. Pengyu Geng, Jinhui Zhao, et al. "Z-Ligustilide Combined with Cisplatin Reduces PLPP1-Mediated Phospholipid Synthesis to Impair Cisplatin Resistance in Lung Cancer." Int. J. Mol. Sci. 2023, 24, 17046.
| Components | 1 mL | 5*1 mL |
| Oligo (dT) 25 Beads (10 mg/mL) | 1 mL(10 mg) | 5*1 mL(50 mg) |
Store at 4 °C for 12-18 months, please DO NOT freeze | ||