RNase Inhibitor, Murine (200 U/μL)

RNase Inhibitor, Murine is a 50 kDa recombinant protein isolated from Escherichia coli, encoded by the murine ribonuclease inhibitor gene. This product exhibits specific inhibitory activity against RNases A, B and C, but does not inhibit RNase 1, RNase T1, S1 nuclease, RNase H, or RNases derived from Aspergillus. It binds to a variety of RNases non-covalently at a molar ratio of 1:1 with high affinity. Notably, previous studies have confirmed that no inhibitory effect of this RNase Inhibitor on enzyme activity is observed when used in combination with AMV or M-MuLV reverse transcriptase, Taq DNA polymerase, or SP6/T7/T3 RNA polymerase. This indicates that it can be applied in diverse experiments to prevent RNA degradation.

Compared with human RNase inhibitor, murine RNase inhibitor lacks a pair of cysteine residues that are highly susceptible to oxidation and cause inhibitor inactivation. Accordingly, murine RNase inhibitor displays markedly improved oxidation resistance relative to human- or porcine-derived RNase inhibitors. In addition, it remains stable at low DTT concentrations (<1 mM), making it particularly suitable for reactions containing low DTT levels (e.g. real-time RT-PCR).

This product can be applied in various reactions to prevent RNA degradation, including RT-PCR, cDNA synthesis, in vitro transcription, enzymatic RNA labeling reactions, and other relevant applications. This product is supplied at a concentration of 200 U/μL. For lower concentration specifications, K1046 (40 U/μL) is recommended.