HyperScribe™ T7 High Yield RNA Synthesis Kit Plus

HyperScribe™ T7 High Yield RNA Synthesis Kit Plus performs in vitro RNA transcription experiment in a relatively short time using T7 RNA polymerase. The kit is designed for high yields of RNA transcripts, highly specific radiolabeled RNA probes and to obtain capped, dye-labeled or biotinylated RNA incorporated modified nucleotides.

RNA synthesized using this kit covers a wide range of applications, such as in vitro translation, antisense RNA and RNAi experiments, RNA vaccines, RNA structure and function studies, ribozyme biochemistry, RNase protein experiments and probe-based hybridization blots.

The kit contains sufficient reagents to carry out 25/50/100 reactions, 20 μL each time. Up to 100 μg of RNA can be generated with 1 μg of control template per standard reaction. Each 25/50/100 reactions kit can produce up to 4.5 mg/9 mg/18 mg RNA.

Figure 1: Transcription using T7 RNA Polymerase

Figure 2: PCR primer design for T7 RNA Polymerase template