Pseudo-UTP
Catalog No.
B7972
Pseudo-modified uridine triphosphate, can replace UTP for in vitro mRNA transcription synthesis.
Featured Products
Pseudo-UTP is a nucleoside triphosphate analogue in which the uracil base is replaced by pseudouracil (pseudouridine). Pseudouridine is a naturally occurring nucleotide modification widely present in various RNA molecules. In vitro transcription reactions can utilize pseudo-UTP instead of UTP to produce RNA molecules containing pseudouridine modifications, enhancing RNA stability and functionality, and thus increasing their persistence within cells. Incorporation of pseudouridine can also enhance RNA translation efficiency and reduce immunogenicity, making it highly valuable for mRNA vaccine development and gene therapy applications.
- 1. Xiaoqing Guan, Hansam Cho, et al. "Omicron KP. 3 RBD-Containing Spike mRNA Vaccine Induces Broadly Neutralizing Antibodies with Protection Against SARS-CoV-2 Omicron Infection in Mice." Vaccines (Basel). 2026 Jan 11;14(1):78. PMID: 41600994
- 2. Qian Liu, Abhishek K. Verma, et al. "MERS-CoV RBD-mRNA Presents Better Immunogenicity and Protection than the Spike-mRNA." Cells. 2025 Dec 4;14(23):1928. PMID: 41369417
- 3. Jeremy J R McIntyre, Connor A Horton, et al. "Different repair pathways support intact or truncated insertions by R2 retrotransposon protein." Science. 2025 Nov 13:eadz3121. PMID: 41231928
- 4. William Stewart, Bin Hu, et al. "A combinatorial siRNA and mRNA approach for obesity treatment using targeting lipid nanoparticles." J Control Release. 2025 Jul 10:383:113857. PMID: 40383157
- 5. Hanjun Li,Jing Lu, et al. "In Situ Delivery of Gasdermin E mRNA Promotes Antitumor Immunity via Creatine-Elicited Type I Interferon Signaling in Monocytes." Cancer Immunol Res. 2025 Jun 4;13(6):939-956. PMID: 40168141
- 6. Xiaoqing Guan, Abhishek K Verma, et al. "A Unique mRNA Vaccine Elicits Protective Efficacy against the SARS-CoV-2 Omicron Variant and SARS-CoV." Vaccines (Basel). 2024 Jun 1;12(6):605. PMID: 38932334
- 7. Jintao Li, et al. "Enhancement of SARS-CoV-2 mRNA Vaccine Efficacy through the Application of TMSB10 UTR for Superior Antigen Presentation and Immune Activation." Vaccines (Basel). 2024 Apr 17;12(4):432. PMID: 38675814
- 8. Jing Lu, Shudan Tan, et al. "Effectiveness of a broad-spectrum bivalent mRNA vaccine against SARS-CoV-2 variants in preclinical studies." Emerg Microbes Infect. 2024 Dec;13(1):2321994. PMID: 38377136
- 9. Mingzhu Gao, Yan Li, et al. "Targeted mRNA Nanoparticles Ameliorate Blood−Brain Barrier Disruption Postischemic Stroke by Modulating Microglia Polarization." ACS Nano. 2024 Jan 30;18(4):3260-3275. PMID: 38227975
- 10. Xingdi Cheng, Sujia Liu, et al. "A Synergistic Lipid Nanoparticle Encapsulating mRNA Shingles Vaccine Induces Potent Immune Responses and Protects Guinea Pigs from Viral Challenges." Adv Mater. 2024 Mar;36(13):e2310886. PMID: 38145557
- 11. Mingzhu Gao, Maoping Tang, et al. "Modulating Plaque Inflammation via Targeted mRNA Nanoparticles for the Treatment of Atherosclerosis." ACS Nano. 2023 Sep 26;17(18):17721-17739. PMID: 37669404
- 12. Fengqiao Li, Xue-Qing Zhang, et al. "mRNA lipid nanoparticle-mediated pyroptosis sensitizes immunologically cold tumors to checkpoint immunotherapy." Nat Commun. 2023 Jul 15;14(1):4223. PMID: 37454146
- 13. Wanbo Tai, Jian Zheng, et al. "MERS-CoV RBD-mRNA vaccine induces potent and broadly neutralizing antibodies with protection against MERS-CoV infection." Virus Res. 2023 Sep:334:199156. PMID: 37336390
- 14. Gang Wang, Juan Shi, et al. "mRNA vaccines elicit potent neutralization against multiple SARS-CoV-2 omicron subvariants and other variants of concern." iScience. 2022 Dec 22;25(12):105690. PMID: 36471872
- 15. Qiyao Chai, Shanshan Yu, et al. "A bacterial phospholipid phosphatase inhibits host pyroptosis by hijacking ubiquitin." Science. 2022 Oct 14;378(6616):eabq0132. PMID: 36227980
- 16. Kyusik Q Kim, Bhagyashri D Burgute, et al. "N1-methylpseudouridine found within COVID-19 mRNA vaccines produces faithful protein products." Cell Rep. 2022 Aug 30;40(9):111300. PMID: 35988540
- 17. Yao Li, Xiaotu Ma, et al. "Rapid Surface Display of mRNA Antigens by BacteriaDerived Outer Membrane Vesicles for a Personalized Tumor Vaccine." Adv Mater. 2022 May;34(20):e2109984. PMID: 35315546
- 18. Jingjing Zhang, Yun Li, et al. "Genome-wide CRISPR/Cas9 library screen identifies PCMT1 as a critical driver of ovarian cancer metastasis." J Exp Clin Cancer Res. 2022 Jan 15;41(1):24. PMID: 35033172
- 19. Wei Wang, Shuo Feng, et al. "Prediction of lipid nanoparticles for mRNA vaccines by the machine learning algorithm." Acta Pharm Sin B
- 20. Shudan Tan, Xue Hu, et al. "Preclinical evaluation of RQ3013, a broad-spectrum mRNA vaccine against SARS-CoV-2 variants." biorxiv
- 21. Trine I Jensen, Nanna S Mikkelsen, et al. "Targeted regulation of transcription in primary cells using CRISPRa and CRISPRi." Genome Res. 2021 Nov;31(11):2120-2130. PMID: 34407984
- 22. Cecilia Martinez Campos, Kevin Tsai, et al. "Mapping of pseudouridine residues on cellular and viral transcripts using a novel antibody-based technique." RNA. 2021 Nov;27(11):1400-1411. PMID: 34376564
- 23. Jing Lu, Guoliang Lu, et al. "A COVID-19 mRNA vaccine encoding SARS-CoV-2 virus-like particles induces a strong antiviral-like immune response in mice." Cell Res. 2020;1-4 PMID: 32801356
| Storage | Store at -20°C or below |
| M.Wt | 484.1 (free acid) |
| Cas No. | 1175-34-4 |
| Formula | C9H15N2O15P3 (free acid) |
| Chemical Name | lithium (5-(2,4-dioxo-1,2,3,4-tetrahydropyrimidin-5-yl)-3,4-dihydroxytetrahydrofuran-2-yl)methyl triphosphate |
| SDF | Download SDF |
| Canonical SMILES | O=C(NC=C1C2C(O)C(O)C(COP([O-])(OP([O-])(OP([O-])([O-])=O)=O)=O)O2)NC1=O.[Li+].[Li+].[Li+].[Li+] |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
Quality Control & MSDS
- View current batch:
-
Purity = 98.73% by AX-HPLC
- COA (Certificate Of Analysis)
- HPLC
- MS (Mass Spectrometry)
- MSDS (Material Safety Data Sheet)
Chemical structure
Related Biological Data
