In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Pseudo-UTP, also known as pseudouridine-5’-O-triphosphate, is one of the most commonly used modified nucleoside-5’-O-triphosphates. Pseudo-UTP is a building block for the polymerase-mediated synthesis of RNA molecules. RNA molecules, especially messenger RNAs (mRNAs), have widespread applications in therapeutic and diagnostic areas, and have demonstrated their superiority over plasmid DNA techniques in both gene and vaccination therapy. Compared with uridine-containing unmodified mRNAs, the application of pseudouridine-containing modified mRNAs exhibits better nuclease stability, immunogenicity, and translational properties. Thus, pseudo-UTP could be used to develop drugable mRNAs with improved properties, by replacing the standard bases by modified ones.
1. Shanmugasundaram M, Senthilvelan A, Kore AR. Gram-scale chemical synthesis of base-modified ribonucleoside-5'-O-triphosphates. Current Protocols in Nucleic Acid Chemistry, 2016, 67: 13.15.1-13.15.10.
|Storage||Store at -20°C or below|
|M.Wt||484.1 (free acid)|
|Formula||C9H15N2O15P3 (free acid)|
|Synonyms||Pseudouridine-5'-Triphosphate, 5-Ribosyl Uracil|
|Chemical Name||lithium (5-(2,4-dioxo-1,2,3,4-tetrahydropyrimidin-5-yl)-3,4-dihydroxytetrahydrofuran-2-yl)methyl triphosphate|
|Shipping Condition||Ship with blue ice, or upon other requests.|
|General tips||For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. We do not recommend long-term storage for the solution, please use it up soon.|