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RNA

Custom mRNA Synthesis

Affordable Cost, High Yields

APExBIO offers affordable custom synthesis of mRNA and long RNA (up to multiple kilobases) with a wide array of modification services at scales ranging from micrograms to milligrams. The mRNA can be generated from DNA templates provided by our customers or we can provide a full service from the ground up. We provide ARCA (Cap 0) and EZ Cap (Cap 1) capping or modified nucleotides implication for all our standard mRNA transcripts.

For ordering and more information, please complete this form (mRNA Synthesis). Our support team ([email protected]) will review and provide a quotation soon.

We also provide LNP formulation and service for lipid based mRNA delivery. 

Modified Nucleotide-containing mRNA Synthesis

Our custom synthesis mRNA covers a wide range of applications:

  • mRNA for genome editing, e.g. Zinc-finger Nuclease mRNA, TALEN mRNA, Cas9 mRNA and Recombinase mRNA.
  • Reporter gene mRNA, such as EGFP mRNA and Luc mRNA, for fluorescence microscopy, flow cytometry and bioluminescent imaging.
  • Reprogramming mRNA, i.e mRNA for non-integrating generation of iPSC.

Validation:

Related Products
Cat.No. Product Name Cat.No. Product Name
B8175 ARCA B8174 mCAP
B7972 Pseudo-UTP B7967 5-Methyl-CTP
B8061 5-Methoxy-UTP K1046 RNase Inhibitor, Murine
K1047 HyperScribe™ T7 High Yield RNA Synthesis Kit K1053 HyperScribe™ Poly (A) Tailing Kit
K1060 HyperScribe™ T7 High Yield Fluorescein RNA Labeling Kit K1061 HyperScribe™ T7 High Yield Cy3 RNA Labeling Kit
K1062 HyperScribe™ T7 High Yield Cy5 RNA Labeling Kit K1063 HyperScribe™ All in One mRNA Synthesis Kit (ARCA, T7, poly(A))
K1064 HyperScribe™ All in One mRNA Synthesis Kit Plus 1 (ARCA, 5mCTP, ψUTP, T7, poly(A)) K1065 HyperScribe™ All in One mRNA Synthesis Kit Plus 2 (ARCA, 5-moUTP, T7, poly(A))
K1401 HyperScribe™ T7 High Yield RNA Synthesis Kit Plus K1402 HyperScribe™ T7 High Yield Fluorescein RNA Labeling Kit Plus
K1403 HyperScribe™ T7 High Yield Cy3 RNA Labeling Kit Plus K1404 HyperScribe™ T7 High Yield Cy5 RNA Labeling Kit Plus
K1405 HyperScribe™ T7 High Yield Biotin 16 RNA Labeling Kit Plus K1406 HyperScribe™ Co-transcription mRNA Synthesis Kit Plus (ARCA, T7)
K1407 HyperScribe™ Co-transcription mRNA Synthesis Kit Plus (ARCA, 5mCTP, ψUTP, T7) K1408 HyperScribe™ Co-transcription mRNA Synthesis Kit Plus (ARCA, 5-moUTP, T7)

 

mRNA Purification

mRNAs transcribed in vitro by T7 RNA polymerase may contain various contaminants, such as short RNAs produced by abortive initiation events, double-stranded (ds)RNAs generated by self-complementary 3’extension, as well as unincorporated nucleoside triphosphates, small abortive transcripts and plasmid template. Certain RNA sequences even induce high levels immunogenicity.

APExBIO offers purification service to remove the contaminants of modified nucleotide-containing mRNA, thus increase the processing efficiency for downstream applications.

Silica-gel Membrane Spin Column Purification:

It is a solid phase extraction technique for fast nucleic acid purification. mRNA can be bound to solid phase of silica-gel membranes under certain conditions, with subsequent washing and elution steps in water or TE pH 7. This method eliminates most proteins, DNA and NTPs.

HPLC purification by ÄKTA avant system:

mRNA can be purified by HPLC (ÄKTA avant system) using column matrix of alkylated non-porous polystyrene-divinylbenzene copolymer microspheres and optimized buffer system, followed by mRNA analyses and mRNA isolation from column fractions.

HPLC purification removes dsRNA and other contaminants from in vitro synthesized modified nucleotide-containing mRNAs, yielding mRNA with the high level of translation without generation of immunogenicity or RNA sensor activation.

mRNA and long RNA products

APExBIO supplies the best quality mRNA and long RNA. This new product lines involve custom synthesis of mRNA and long RNA (up to multiple kilobases) with a wide array of modification services at scales ranging from micrograms to milligrams. The mRNA can be generated from DNA templates provided by our customers or we can provide a full service from the ground up. We offer ARCA (Cap 0) or EZ Cap (Cap 1) modified nucleotides implication for all our standard mRNA transcripts.

All of our mRNA products offer:

  • Incorporates ARCA (Cap 0) or EZ Cap (Cap 1) into the transcript to increase translation efficiency
  • Reduces host cell immune response and enhances stability by incorporating modified nucleotides (5mCTP and ψUTP) and a poly(A) tail
  • Degrades the DNA template after RNA synthesis with DNase
  • Removes the 5’ triphosphates at the end of the RNA with phosphatase to further reduce innate immune responses in mammalian cells
  • Employs a robust clean-up spin column system that delivers high yields of mRNAs that are ready for most downstream applications

Validation:

Related Products
Cat.No. Product Name Cat.No. Product Name
R1001 ARCA EGFP mRNA R1005 Firefly Luciferase mRNA (ARCA, 5mCTP, ψUTP)
R1006 SpCas9 mRNA (ARCA, 5mCTP, ψUTP) R1007 ARCA EGFP mRNA (5-moUTP)
R1008 ARCA Cy3 EGFP mRNA (5-moUTP) R1009 ARCA Cy5 EGFP mRNA (5-moUTP)
R1010 EZ Cap™ Cy5 Firefly Luciferase mRNA (5-moUTP) R1011 EZ Cap™ Cy5 EGFP mRNA (5-moUTP)
R1012 Firefly Luciferase mRNA (ARCA, 5-moUTP) R1013 EZ Cap™ Firefly Luciferase mRNA (5-moUTP)
R1015 EZ Cap™ Cas9 mRNA (5-moUTP) R1016 EZ Cap™ EGFP mRNA (5-moUTP)
R1017 EZ Cap™ mCherry mRNA (5mCTP, ψUTP) R1018 EZ Cap™ Firefly Luciferase mRNA
R1020 EZ Cap™ EPO mRNA (ψUTP) R1021 EZ Cap™ Renilla Luc mRNA
R1022 EZ Cap™ Renilla Luc mRNA (5-moUTP) R1023 EZ Cap™ Human p53 mRNA
R1024 EZ Cap™ Human p53 mRNA (ψUTP) R1025 EZ Cap™ Human PTEN mRNA
R1026 EZ Cap™ Human PTEN mRNA (ψUTP) R1027 EZ Cap™ OVA mRNA
R1028 EZ Cap™ OVA mRNA (5-moUTP) R1029 EZ Cap™ Cre mRNA (ΨUTP)
R1030 EZ Cap™ Cre mRNA (m1Ψ) R1031 Negative EZ Cap™ EGFP mRNA (5-moUTP)
R1032 EZ Cap™ Mouse p53 mRNA R1033 EZ Cap™ Mouse p53 mRNA (ψUTP)
R1034 EZ Cap™ Mouse p53 mRNA (5-moUTP) R1035 EZ Cap™ Fdx1 mRNA
R1036 EZ Cap™ Fdx1 mRNA (ψUTP) R1037 EZ Cap™ Fdx1 mRNA (5-moUTP)