RNA
Custom mRNA Synthesis
Affordable Cost, High Yields
APExBIO offers affordable custom synthesis of mRNA and long RNA (up to multiple kilobases) with a wide array of modification services at scales ranging from micrograms to milligrams. The mRNA can be generated from DNA templates provided by our customers or we can provide a full service from the ground up. We provide ARCA (Cap 0) and EZ Cap (Cap 1) capping or modified nucleotides implication for all our standard mRNA transcripts.
For ordering and more information, please complete this form (mRNA Synthesis). Our support team ([email protected]) will review and provide a quotation soon.
We also provide LNP formulation and service for lipid based mRNA delivery.
Modified Nucleotide-containing mRNA Synthesis
Our custom synthesis mRNA covers a wide range of applications:
- mRNA for genome editing, e.g. Zinc-finger Nuclease mRNA, TALEN mRNA, Cas9 mRNA and Recombinase mRNA.
- Reporter gene mRNA, such as EGFP mRNA and Luc mRNA, for fluorescence microscopy, flow cytometry and bioluminescent imaging.
- Reprogramming mRNA, i.e mRNA for non-integrating generation of iPSC.
Validation:
mRNA Purification
mRNAs transcribed in vitro by T7 RNA polymerase may contain various contaminants, such as short RNAs produced by abortive initiation events, double-stranded (ds)RNAs generated by self-complementary 3’extension, as well as unincorporated nucleoside triphosphates, small abortive transcripts and plasmid template. Certain RNA sequences even induce high levels immunogenicity.
APExBIO offers purification service to remove the contaminants of modified nucleotide-containing mRNA, thus increase the processing efficiency for downstream applications.
Silica-gel Membrane Spin Column Purification:
It is a solid phase extraction technique for fast nucleic acid purification. mRNA can be bound to solid phase of silica-gel membranes under certain conditions, with subsequent washing and elution steps in water or TE pH 7. This method eliminates most proteins, DNA and NTPs.
HPLC purification by ÄKTA avant system:
mRNA can be purified by HPLC (ÄKTA avant system) using column matrix of alkylated non-porous polystyrene-divinylbenzene copolymer microspheres and optimized buffer system, followed by mRNA analyses and mRNA isolation from column fractions.
HPLC purification removes dsRNA and other contaminants from in vitro synthesized modified nucleotide-containing mRNAs, yielding mRNA with the high level of translation without generation of immunogenicity or RNA sensor activation.
mRNA and long RNA products
APExBIO supplies the best quality mRNA and long RNA. This new product lines involve custom synthesis of mRNA and long RNA (up to multiple kilobases) with a wide array of modification services at scales ranging from micrograms to milligrams. The mRNA can be generated from DNA templates provided by our customers or we can provide a full service from the ground up. We offer ARCA (Cap 0) or EZ Cap (Cap 1) modified nucleotides implication for all our standard mRNA transcripts.
All of our mRNA products offer:
- Incorporates ARCA (Cap 0) or EZ Cap (Cap 1) into the transcript to increase translation efficiency
- Reduces host cell immune response and enhances stability by incorporating modified nucleotides (5mCTP and ψUTP) and a poly(A) tail
- Degrades the DNA template after RNA synthesis with DNase
- Removes the 5’ triphosphates at the end of the RNA with phosphatase to further reduce innate immune responses in mammalian cells
- Employs a robust clean-up spin column system that delivers high yields of mRNAs that are ready for most downstream applications
Validation:
