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EZ Cap™ Mito-mTurquoise2 Probe mRNA (m1Ψ)

Catalog No.
R1114
Mitochondria (Mito) probes mRNA with cyan fluorescent protein (mTurquoise2), modified by N1-Methylpseudo-UTP (m1Ψ), providing higher transcription efficiency and suppressing RNA-mediated innate immune activation.
Grouped product items
SizePriceStock Qty
100ug(1mg/mL)
$210.00
In stock
1mg(1mg/mL)
$1,136.00
In stock
5x1mg(1mg/mL)
$4,090.00
In stock
For scientific research use only and should not be used for diagnostic or medical purposes.

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

Background

Mito-mTurquoise2 is a fusion protein, consisting of Mitochondria (Mito) targeting sequence and cyan fluorescent protein (mTurquoise2). mTurquoise2 has high brightness and stability, and is suitable for live cell imaging. By fusing mTurquoise2 with mitochondrial signal sequences, the dynamic changes of Mito can be observed in real time in living cells, helping to reveal the key role of Mito in cell metabolism, signal transduction and apoptosis. Mito-mTurquoise2 is an ideal tool for the study of mitochondrial morphology and function due to its efficient fluorescence properties and mitochondria-specific localization.

EZ Cap™ Mito-mTurquoise2 Probe mRNA (m1Ψ) is provided at a concentration of ~1 mg/ml with Cap1 structure. It is specifically used for the expression of cyan fluorescent protein markers in Mito. There are currently two ways to cap mRNA: One is co-transcription method, by adding Cap analogues into the transcription process. The other is enzymatic Capping. After transcription, Cap0 capping is performed by Vaccinia virus Capping Enzyme (VCE), GTP and S-adenosylmethionine (SAM). The Cap0 is then generated into the Cap1 through 2´-O-Methyltransferase and SAM. Cap1 Capping can also be performed by adding VCE, 2´-O-Methyltransferase, GTP and SAM in a one-step process. Cap 1 structure is more ideal for mammalian systems and possess higher transcription efficiency than Cap 0 structure. The addition of N1-Methylpseudo-UTP(m1Ψ) and poly(A) tail suppress RNA-mediated innate immune activation and increase the stability and lifetime of the mRNA in vitro and in vivo. Poly(A) tail also plays an important role in enhancing the efficiency of translation initiation.

Quality Control

Quality Control & MSDS

View current batch:
 

Description

mRNA Length

1080 nucleotides

Concentration

~1 mg/mL

Excitation max (nm)

434 nm (mTurquoise2)

Emission max (nm)

474 nm (mTurquoise2)

Buffer

1 mM Sodium Citrate, pH 6.4

Storage

-40°C or below

General tips

Please dissolve it on ice and protect from RNase carefully. Avoid repeated freeze/thaw cycles as possible. Don’t vortex. Upon first use, centrifuge the tube softly and aliquot it into several single use portions. Use RNase-free reagents and materials with appropriate RNase-free technique. Don’t add to the media with serum unless mixing with a transfection reagent.

Shipping Condition

ship with dry ice

Biological Activity