EZ Cap™ Nuc-mScarlet Probe mRNA (m1Ψ)

Nuc-mScarlet is a fusion protein, that combines Nucleus (Nuc) localization signal and red fluorescent protein (mScarlet). mScarlet is widely used in biological imaging due to its high brightness and rapid maturation. By fusing mScarlet with nuclear localization signals, the dynamic processes within the nucleus can be observed in real time in living cells. The high light stability and brightness of Nuc-mScarlet make it suitable for long-term imaging experiments. In addition, mScarlet's spectral properties are compatible with other fluorescent proteins such as GFP, facilitating multi-labeling experiments.

EZ Cap™ Nuc-mScarlet Probe mRNA (m1Ψ) is provided at a concentration of ~1 mg/ml with Cap1 structure. It is specifically used for the expression of red fluorescent protein markers in Nucleus. There are currently two ways to cap mRNA: One is co-transcription method, by adding Cap analogues into the transcription process. The other is enzymatic Capping. After transcription, Cap0 capping is performed by Vaccinia virus Capping Enzyme (VCE), GTP and S-adenosylmethionine (SAM). The Cap0 is then generated into the Cap1 through 2´-O-Methyltransferase and SAM. Cap1 Capping can also be performed by adding VCE, 2´-O-Methyltransferase, GTP and SAM in a one-step process. Cap 1 structure is more ideal for mammalian systems and possess higher transcription efficiency than Cap 0 structure. The addition of N1-Methylpseudo-UTP(m1Ψ) and poly(A) tail suppress RNA-mediated innate immune activation and increase the stability and lifetime of the mRNA in vitro and in vivo. Poly(A) tail also plays an important role in enhancing the efficiency of translation initiation.