EZ Cap™ Lifeact-mTurquoise2 Probe mRNA (m1Ψ)

Lifeact-mturquoise2 is a fusion protein, consisting of Lifeact peptide and cyan fluorescent protein mTurquoise2. Lifeact peptides bind specifically to Actin fibers, are often used to label and study actin dynamics in the cytoskeleton. mTurquoise2 has high brightness and stability, and is suitable for live cell imaging. By fusing mTurquoise2 with Lifeact peptide sequence, the distribution and dynamic changes of actin can be observed in real time in living cells, revealing the cytoskeletal recombination process involving cell migration, division and morphological changes.

EZ Cap™ Lifeact-mturquoise2 Probe mRNA (m1Ψ) is provided at a concentration of ~1 mg/ml with Cap1 structure. It is specifically used for the expression of cyan fluorescent protein markers in cytoskeleton. There are currently two ways to cap mRNA: One is co-transcription method, by adding Cap analogues into the transcription process. The other is enzymatic Capping. After transcription, Cap0 capping is performed by Vaccinia virus Capping Enzyme (VCE), GTP and S-adenosylmethionine (SAM). The Cap0 is then generated into the Cap1 through 2´-O-Methyltransferase and SAM. Cap1 Capping can also be performed by adding VCE, 2´-O-Methyltransferase, GTP and SAM in a one-step process. Cap 1 structure is more ideal for mammalian systems and possess higher transcription efficiency than Cap 0 structure. The addition of N1-Methylpseudo-UTP(m1Ψ) and poly(A) tail suppress RNA-mediated innate immune activation and increase the stability and lifetime of the mRNA in vitro and in vivo. Poly(A) tail also plays an important role in enhancing the efficiency of translation initiation.