EZ Cap™ EPO mRNA (ψUTP)

The human erythropoietin (EPO) protein encoded by EPO mRNA is a hormone that controls erythropoiesis or erythropoiesis. EPO also plays a role in wound healing and the brain's response to nerve damage. Studies have shown that transfection of EPO mRNA in vivo leads to a significant increase in reticulocyte and hematocrit measurement.

EZ Cap™ EPO mRNA (ψUTP) is provided at a concentration of ~1 mg/ml with Cap1 structure. There are currently two ways to cap mRNA: One is co-transcription method, by adding Cap analogues into the transcription process. The other is enzymatic Capping. After transcription, Cap0 capping is performed by Vaccinia virus Capping Enzyme (VCE), GTP and S-adenosylmethionine (SAM). The Cap0 is then generated into the Cap1 through 2´-O-Methyltransferase and SAM. Cap1 Capping can also be performed by adding VCE, 2´-O-Methyltransferase, GTP and SAM in a one-step process. Cap 1 structure is more ideal for mammalian systems and possess higher transcription efficiency than Cap 0 structure. The addition of ψUTP and poly(A) tail suppress RNA-mediated innate immune activation and increase the stability and lifetime of the mRNA in vitro and in vivo. Poly(A) tail also plays an important role in enhancing the efficiency of translation initiation.