RNA Clean and Concentrator Kit
The RNA Clean and Concentrator Kit is suitable for rapid, high-throughput purification of RNA obtained from enzymatic reactions (e.g., in vitro transcription). This kit efficiently separates RNA from unincorporated NTPs, enzymes, and reaction components, making it optimal for rapid cleanup of large-scale transcription reactions. It can also effectively remove nucleotides, short oligonucleotides, proteins, and salts from RNA samples.
The procedure is simple and rapid, offering effective recovery of RNA ranging from 1 ng to 500 μg. It is ideal for purifying single-stranded RNA greater than 100 nucleotides and double-stranded RNA greater than 200 base pairs. The RNA Clean and Concentrator Kit requires only three steps: binding RNA onto the membrane in the spin column, washing away contaminants, and eluting purified RNA in a low-salt buffer. The recovered RNA is suitable for downstream applications requiring highly pure RNA.
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- 2. H He, Y Tang, et al. "PINK1/Park2-mediated mitophagy relieve non-alcoholic fatty liver disease." Physiol Res. 2024 Apr 30;73(2):253-263. PMID: 38710055
- 3. Jing Lu, Guoliang Lu, et al. "A COVID-19 mRNA vaccine encoding SARS-CoV-2 virus-like particles induces a strong antiviral-like immune response in mice." Cell Res. 2020;1-4. PMID:32801356
| Components | Amount | Storage |
| Binding Solution | 8 mL | 4°C |
| Wash Solution Concentrate (Add 20 mL 100% ethanol before use) |
5 mL | 4°C |
| 5 M Ammonium Acetate | 1 mL | 4°C |
| Elution Solution | 5 mL | -20°C, 4°C, or room temperature |
| Filter Cartridges & Collection Tubes | 20 | room temperature |
| Collection and Elution Tubes | 20 | room temperature |