In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
A critical step in mRNA processing is the addition of a 5’ cap structure, a 5'-5' triphosphate linkage between the 5' initiating terminal of the RNA and a guanosine nucleotide. The cap is then methylated enzymatically at the N-7 position of the guanosine to form a mature mCAP.
And now, Anti Reverse Cap Analog (ARCA) is introduced. ARCA with the ability only inserting in the proper orientation, results in forming mRNAs that can be translated twice efficiently as those initiated with mCAP.
When preparing synthetic mRNA, the cap is often added prior to use in order to stabilize the mRNA and significantly enhance translation. Using a 4:1 mixture of a cap analog to GTP in transcription reactions will cap 80% of the resulting mRNAs.
. Ramanathan A1, Robb GB1, et al, mRNA capping: biological functions and applications. Nucleic Acids Res. 2016 Sep 19;44(16):7511-26.
. Guo XR1,2, Wang XL2, et al, PDX-1 mRNA-induced reprogramming of mouse pancreas-derived mesenchymal stem cells into insulin-producing cells in vitro. Clin Exp Med. 2015 Nov;15(4):501-9.
. Lee K1, Yu P2,et al, Peptide-enhanced mRNA transfection in cultured mouse cardiac fibroblasts and direct reprogramming towards cardiomyocyte-like cells. Int J Nanomedicine. 2015 Mar 6;10:1841-54.
. Kenney SP1, Meng XJ2. The lysine residues within the human ribosomal protein S17 sequence naturally inserted into the viral nonstructural protein of a unique strain of hepatitis E virus are important for enhanced virus replication. J Virol. 2015 Apr;89(7):3793-803.
. Almåsbak H1, Walseng E2,et al, Inclusion of an IgG1-Fc spacer abrogates efficacy of CD19 CAR T cells in a xenograft mouse model. Gene Ther. 2015 May;22(5):391-403.
|Storage||Store at -20°C or below|
|M.Wt||817.4 (free acid)|
|Formula||C22H32N10O18P3 (free acid)|
|Synonyms||Anti Reverse Cap Analog, 3´-O-Me-m7G(5')ppp(5')G|
|Shipping Condition||Ship with blue ice, or upon other requests.|
|General tips||For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. We do not recommend long-term storage for the solution, please use it up soon.|