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HyperScribe™ SP6 High Yield RNA Synthesis Kit

Catalog No.
K1415
For high-efficiency in vitro transcription
Grouped product items
SizePriceStock Qty
25rxns
$120.00
In stock
50rxns
$200.00
In stock
100rxns
$370.00
In stock
For scientific research use only and should not be used for diagnostic or medical purposes.

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

Product Citation

Description

HyperScribe™ SP6 High Yield RNA Synthesis Kit performs in vitro RNA transcription experiment in a relatively short time using SP6 RNA polymerase. The kit is designed for high yields of RNA transcripts, highly specific radiolabeled RNA probes and to obtain capped, dye-labeled or biotinylated RNA incorporated modified nucleotides.

RNA synthesized using this kit covers a wide range of applications, such as in vitro translation, antisense RNA and RNAi experiments, RNA vaccines, RNA structure and function studies, ribozyme biochemistry, RNase protein experiments and probe-based hybridization blots.

The kit contains sufficient reagents to carry out 25/50/100 reactions, 20 μL each time. ≥50 μg of RNA can be generated with 1 μg of control template per standard reaction.

Quality Control

Quality Control & DataSheet

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Components and Storage

Components 25 rxns 50 rxns 100 rxns
SP6 RNA Polymerase Mix 50 μL 100 μL 200 μL
10 × Reaction Buffer 50 μL 100 μL 200 μL
ATP (100 mM) 25 μL 50 μL 100 μL
GTP (100 mM) 25 μL 50 μL 100 μL
UTP (100 mM) 25 μL 50 μL 100 μL
CTP (100 mM) 25 μL 50 μL 100 μL
Control Template (0.5 μg/μL) 5 μL 10 μL 20 μL
RNase-free H2O 0.5 mL 1 mL 2 x 1 mL
DNase I (RNase-free) (2 U/µl) 25 μL 50 μL 100 μL

Store all the kit components at -20℃.