HyperScribe™ T7 High Yield Cy5 RNA Labeling Kit
HyperScribe™ T7 High Yield Cy5 RNA Labeling Kit is designed to generate randomly Cy5-modified RNA probes by in vitro transcription. Such probes are ideally suited for in situ hybridization and Northern blot hybridization experiments. The principle of labeling is similar to the basic labeling principle of a mixture of Cy5 RNA labels. Cy5-UTP is efficiently incorporated into RNA using the optimized reaction buffer and T7 RNA polymerase mixture in place of its natural counterpart UTP. An appropriate Cy5-UTP substitution typically achieves an optimal balance between reaction and labeling efficiency. However, the individual optimization of the Cy5-UTP/UTP ratio can be easily achieved using a single nucleotide format. The resulting Cy5 -modified RNA probe can then be detected by fluorescence spectroscopy.
An upgraded version of this product is available with higher yield (~100ug), please check out K1404 HyperScribe™ T7 High Yield Cy5 RNA Labeling Kit Plus for more details.- 1. Su He, Hongwei Gou, et al. "The SARS-CoV-2 nucleocapsid protein suppresses innate immunity by remodeling stress granules to atypical foci." FASEB J. 2023 Dec;37(12):e23269 PMID: 37889852
- 2. Lara A Gruijs da Silva, Francesca Simonetti, et al. "Disease-linked TDP-43 hyperphosphorylation suppresses TDP-43 condensation and aggregation." EMBO J. 2022 Apr 19;41(8):e108443 PMID: 35112738
- 3. Weiqi Cai, Tianli Luo, et al. "A Combinatorial Library of Biodegradable Lipid Nanoparticles Preferentially Deliver mRNA into Tumor Cells to Block Mutant RAS Signaling." Adv. Funct. Mater. 2022, 32, 2204947
- 4. Ming Zhao, Yu Yu, et al. "GCG inhibits SARS-CoV-2 replication by disrupting the liquid phase condensation of its nucleocapsid protein." Nat Commun. 2021 Apr 9;12(1):2114. PMID: 33837182
| Components | 25 rxns |
| T7 RNA Polymerase Mix | 50 uL |
| 10X Reaction Buffer | 50 uL |
| ATP (20 mM) | 50 uL |
| GTP (20 mM) | 50 uL |
| UTP (20 mM) | 37.5 uL |
| CTP (20 mM) | 50 uL |
| Cy5-UTP (10 mM) | 25 uL |
| Control Template (0.5 ug/uL) | 5 uL |
| RNase-free H2O | 1 mL |
Store all the kit components at -20°C. | |
