HyperScribe™ T7 High Yield Cy5 RNA Labeling Kit
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Components and Storage
|T7 RNA Polymerase Mix||50 uL|
|10X Reaction Buffer||50 uL|
|ATP (20 mM)||50 uL|
|GTP (20 mM)||50 uL|
|UTP (20 mM)||37.5 uL|
|CTP (20 mM)||50 uL|
|Cy5-UTP (10 mM)||25 uL|
|Control Template (0.5 ug/uL)||5 uL|
|RNase-free H2O||1 mL|
Store all the kit components at -20°C.
HyperScribe™ T7 High Yield Cy5 RNA Labeling Kit is designed to generate randomly Cy5-modified RNA probes by in vitro transcription. Such probes are ideally suited for in situ hybridization and Northern blot hybridization experiments. The principle of labeling is similar to the basic labeling principle of a mixture of Cy5 RNA labels. Cy5-UTP is efficiently incorporated into RNA using the optimized reaction buffer and T7 RNA polymerase mixture in place of its natural counterpart UTP. An appropriate Cy5-UTP substitution typically achieves an optimal balance between reaction and labeling efficiency. However, the individual optimization of the Cy5-UTP/UTP ratio can be easily achieved using a single nucleotide format. The resulting Cy5 -modified RNA probe can then be detected by fluorescence spectroscopy.