High Yield T7 Biotin16 RNA Labeling Kit
- 1. Jing-Lan Hao, Jia-Qi He, et al. "TLL1 knockdown attenuates prostate cancer progression by enhancing antitumor immunity." bioRxiv. November 12, 2024.
- 2. Honglin Liu, Zhenyu Han, et al. "ZNFX1 promotes AMPK-mediated autophagy against Mycobacterium tuberculosis by stabilizing Prkaa2 mRNA." JCI Insight. 2024 Jan 9;9(1):e171850. PMID: 38016036
High Yield T7 Biotin16 RNA Labeling Kit is designed to generate randomly Biotin16-modified RNA probes by in vitro transcription. Such probes are ideally suited for in situ hybridization and Northern blot hybridization experiments. The principle of labeling is similar to the basic labeling principle of a mixture of Biotin16 RNA labels. Biotin16-UTP is efficiently incorporated into RNA using the optimized reaction buffer and T7 RNA polymerase mixture in place of its natural counterpart UTP. An appropriate Biotin16-UTP substitution typically achieves an optimal balance between reaction and labeling efficiency. However, the individual optimization of the Biotin16-UTP/UTP ratio can be easily achieved using a single nucleotide format. The resulting Biotin-modified RNA probe can then be detected by fluorescence spectroscopy.
An upgraded version of this product is available with higher yield (~100ug), please check out K1405 HyperScribe™ T7 High Yield Biotin 16 RNA Labeling Kit Plus for more details.| Components | 25 rxns |
| T7 RNA Polymerase Mix | 50 μL |
| 10X Reaction Buffer | 50 μL |
| ATP (20 mM) | 50 μL |
| GTP (20 mM) | 50 μL |
| UTP (20 mM) | 37.5 μL |
| CTP (20 mM) | 50 μL |
| Biotin16-UTP (10mM) | 25 μL |
| Control Template(0.5 μg/μL) | 5 μL |
| RNase-free H2O | 1 mL |
Store all the kit components at -20°C. | |