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High Yield T7 Biotin16 RNA Labeling Kit

Catalog No.
K1082
 
Grouped product items
SizePriceStock Qty
25 rxns
$200.00
In stock
For scientific research use only and should not be used for diagnostic or medical purposes.

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

Product Citation

Description

High Yield T7 Biotin16 RNA Labeling Kit is designed to generate randomly Biotin16-modified RNA probes by in vitro transcription. Such probes are ideally suited for in situ hybridization and Northern blot hybridization experiments. The principle of labeling is similar to the basic labeling principle of a mixture of Biotin16 RNA labels. Biotin16-UTP is efficiently incorporated into RNA using the optimized reaction buffer and T7 RNA polymerase mixture in place of its natural counterpart UTP. An appropriate Biotin16-UTP substitution typically achieves an optimal balance between reaction and labeling efficiency. However, the individual optimization of the Biotin16-UTP/UTP ratio can be easily achieved using a single nucleotide format. The resulting Biotin-modified RNA probe can then be detected by fluorescence spectroscopy.

An upgraded version of this product is available with higher yield (~100ug), please check out K1405 HyperScribe™ T7 High Yield Biotin 16 RNA Labeling Kit Plus for more details.

Quality Control

Quality Control & DataSheet

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Components and Storage

Components 25 rxns
T7 RNA Polymerase Mix 50 μL
10X Reaction Buffer 50 μL
ATP (20 mM) 50 μL
GTP (20 mM) 50 μL
UTP (20 mM) 37.5 μL
CTP (20 mM) 50 μL
Biotin16-UTP (10mM) 25 μL
Control Template(0.5 μg/μL) 5 μL
RNase-free H2O 1 mL

Store all the kit components at -20°C.

Storage

Store all the kit components at -20°C.