High Yield T7 Biotin16 RNA Labeling Kit

High Yield T7 Biotin 16 RNA Labeling Kit is designed to generate randomly Biotin-modified RNA probes by in vitro transcription. Such probes are ideally suited for in situ hybridization and Northern blot hybridization experiments. The principle of labeling is similar to the basic labeling principle of a mixture of Biotin RNA labels. Biotin-16-UTP is efficiently incorporated into RNA using the optimized reaction buffer and T7 RNA polymerase mixture in place of its natural counterpart UTP. An appropriate Biotin-16-UTP substitution typically achieves an optimal balance between reaction and labeling efficiency. The resulting Biotin-modified RNA probe can then be detected by HRP- or AP-modified streptavidin.

An upgraded version of this product is available with higher yield (~100ug), please check out K1405 HyperScribe™ T7 High Yield Biotin 16 RNA Labeling Kit Plus for more details.