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Hyper Assembly Cloning Kit

Catalog No.
K1054
used for fast, directional cloning of one or more fragments of DNA into any vector.
Grouped product items
SizePriceStock Qty
25 rxns
$80.00
In stock
50 rxns
$150.00
In stock
100 rxns
$250.00
In stock

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

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Related Biological Data

Hyper Assembly Cloning Kit

Related Biological Data

Hyper Assembly Cloning Kit
 

Protocol

Components and Storage

Component Size Volume Storage

5X Hyper Assembly Enzyme Mix

25 rxns 12.5 μL

Store at -20°C

50 rxns 25 μL
100 rxns 50 μL

Description

Hyper Assembly Cloning Kit is designed for fast, directional cloning of one or more target fragment of DNA into any vector. This method employs the 3’-5’ exonuclease activity of Hyper Assembly Enzyme to get 15-20 nt complementary arms at the ends of PCR-generated inserts and linearized vectors, then fuse the DNA fragments to get the recombinant vectors. These 15-20 bp overlaps can be engineered by designing primers for amplification of the desired sequences.

• Clone any insert into any location within any vector you choose

• Efficiently clone a broad range of fragment sizes

• Clone multiple DNA fragments simultaneously into any vector in a single reaction

• No restriction digestion, phosphatase treatment, or ligation required

Storage

Store at -20°C.