Taq DNA Ligase

Taq DNA Ligase is a thermostable ligase that catalyzes the formation of phosphodiester bonds between the 5′-phosphate and 3′-hydroxyl groups of two adjacent oligonucleotide chains that hybridize to the same complementary target DNA strand. This reaction occurs only when the two oligonucleotide chains are perfectly paired with the target DNA with no gaps, making it suitable for single-base substitution detection. Taq DNA Ligase uses NAD⁺ as a cofactor and remains active within a temperature range of 45–65℃.