2X UltraFidelity™ Master Mix with dye

2X UltraFidelity™ Master Mix with dye is an efficient and convenient premix specifically designed for high-fidelity DNA amplification. It employs a thermostable DNA polymerase with 3'→5' exonuclease activity and incorporates the Sso7d DNA domain to enhance continuous synthesis capability, ensuring the stability of the DNA amplification process. The error rate of this enzyme is as low as 1/280 that of Taq DNA polymerase, making it particularly suitable for cloning experiments involving long fragments or difficult-to-amplify sequences.

2×UltraFidelity™ Master Mix with dye contains polymerase, dNTPs, buffer, and dye. When diluted to a 1× concentration, the final concentration of Mg++ is 2 mM. Compared to conventional PCR enzymes, our product provides higher fidelity.

After PCR amplification, the product can be directly subjected to electrophoresis without the need to add additional loading buffer. If the subsequent experiment involves cloning, it is recommended to choose the dye-free version of 2×UltraFidelity™ Master Mix (Cat. No. K1501).