2X Taq PCR Master Mix Plus (with dye)

Taq DNA polymerase can synthesize DNA according to the template under appropriate conditions, in the presence of gene-specific primers and dNTPs. Taq DNA polymerase has the ability of 5'→3'DNA polymerase and a weak 5'→3' exonuclease, but does not have the ability of 3'→5' exonuclease, which means that a dA protrusion will appear at the 3' end. Using this feature, it can be used for TA cloning.

This product contains the modified Taq DNA Polymerase, dNTP and an optimized buffer system. The Taq DNA Polymerase used has strong amplification performance and can efficiently amplify DNA fragments of ≤7 kb. It can achieve efficient amplification of different GC contents (30% - 70%). It is compatible with a variety of complex templates and is suitable for a wide range of applications, including plant and colony PCR as well as mouse gene identification. This Master Mix operation only requires the addition of primers, templates and ddH2O, which greatly simplifies the experimental steps, reduces personal errors and improves the repeatability of the results. This product contains blue dye, which means it can be directly subjected to electrophoresis after amplification without the need to add sample loading buffer. If agarose Gel electrophoresis of PCR products is required, our product SYBR Safe DNA Gel Stain (No. A8743) can be added to the gel.