2X HyperFusion™ High-Fidelity Master Mix

Our product 2X HyperFusion™ High-Fidelity Master Mix offers superior performance for major PCR applications with high fidelity, powerful amplification ability and faster amplification speed. HyperFusion™ high-fidelity DNA polymerases are composed of a DNA-binding domain fused to a Pyrococcus-like proofreading polymerase. Its unique structure, a novel Pyrococcus-like enzyme fused with a processivity-enhancing domain, increases fidelity and speed. The high fidelity makes HyperFusion™ high-fidelity DNA polymerase a superior choice for cloning or other subsequent applications. HyperFusion™ high-fidelity DNA polymerase possesses one of the most accuracy with an error rate that is 50-fold lower compared to Taq DNA Polymerase and 6-fold lower than Pyrococcus Furiosus DNA Polymerase (Pfu).

HyperFusion™ DNA polymerase possesses 5´→ 3´ polymerase activity and 3´→ 5´ exonuclease activity. It will generate blunt-ended products in the amplification products without an A overhang which appears in the product amplified with Taq polymerase. The polymerase has been capable of amplifying fragment as long as 10 kb in our assays. In PCR reaction, elongation rate of HyPerFUsion® DNA polymerase is about 15-30 sec/kb depending on the complexity of the gene.

2X HyperFusion™ High-Fidelity Master Mix is a ready-to-use 2X premix solution containing polymerase, dNTPs and an already optimized buffer system.

High fidelity —up to 50X more accurate than Taq; up to 6X more accurate than Pfu DNA polymerase.

Enhanced robustness—fewer reaction failures and minimal optimization.

Improved yields—high product yields in one reaction.

High speed—increased processivity allows shorter reaction times.

Quick and simply ready-to-use PCR mix.