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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Dpn I is a Dam methylation-sensitive restriction enzyme that recognizes GA/TC sequences, and can only cleave DNA when adenine N6-methylation is present in the sequence. The digestion will be blocked when CpG methylation of mammalian DNA overlaps with restriction sites of Dpn I. In addition, Dpn Ⅰ had no effect on Dcm methylation. This product is suitable for rapid digestion reactions of plasmid DNA, PCR products or genomic DNA, and is widely used in many research fields such as genotyping, molecular cloning, Southern blotting, SNP, RFLP technology, etc.
The restriction sites of Dpn I as follows:
5'…… G Am6 ↓T C…… 3'
3'…… C T↑ Am6G…… 5'
Store the components at -20 °C.