Biotin-16-dCTP
Biotin-16-dCTP is a modified deoxynucleoside triphosphate (dNTP), serving as a convenient tool for the enzymatic introduction of the biotin moiety into a nucleic acid target of interest. The biotin moiety exhibits a very strong affinity for streptavidin. The strength and specificity of this interaction has been exploited in a variety of biological applications, such as secondary label introduction and affinity isolation. For the biotinylated nucleotides, biotin-16-dCTP and biotin-16-dUTP, strong amplicon formation can be observed with modified nucleotides substituted for 75% of their natural counterparts (i.e. dCTP and dUTP). Compared with biotin-16-dUTP whose yield decreases quickly above 75% substitution, the yield of biotin-16-dCTP decreases at a much higher substitution of ∼90%. It turns out that Taq DNA polymerase can incorporate biotin-16-dCTP with greater efficiency than biotin-16-dUTP.
Reference:
1. Natasha P, Joyclyn Y. PCR incorporation of modified dNTPs: the substrate properties of biotinylated dNTPs. BioTechniques, 2010, 48(4): 333 - 334.
| Physical Appearance | Solution |
| Storage | Store at -20°C or below |
| M.Wt | 946.7 (free acid) |
| Formula | C32H53N8O17P3S (free acid) |
| Synonyms | Biotin-16-Aminoallyl-2'-dCTP, Biotin-16-AA-dCTP |
| Chemical Name | lithium (E)-(5-(4-amino-2-oxo-5-(3-(4-(6-(5-(2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)hexanamido)butanamido)prop-1-en-1-yl)pyrimidin-1(2H)-yl)-3-hydroxytetrahydrofuran-2-yl)methyl triphosphate |
| SDF | Download SDF |
| Canonical SMILES | NC(C(/C=C/CNC(CCCNC(CCCCCNC(CCCCC1C(NC(N2)=O)C2CS1)=O)=O)=O)=CN3C4CC(O)C(COP([O-])(OP([O-])(OP([O-])([O-])=O)=O)=O)O4)=NC3=O.[Li+].[Li+].[Li+].[Li+] |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
Quality Control & MSDS
- View current batch:
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Purity = ≥90% by AX-HPLC
- COA (Certificate Of Analysis)
- MSDS (Material Safety Data Sheet)
Chemical structure
