Biotin-16-dUTP
Catalog No.
B8150
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Biotin-16-dUTP is a biotin-labeled nucleotide analog utilized extensively in biomedical research for nucleic acid modification and detection. Structurally modified to contain a biotin moiety linked via a 16-carbon spacer, this compound functions by enzymatic incorporation into DNA strands mediated by DNA polymerases. Following integration, the biotin group serves as an affinity tag, enabling subsequent detection through biotin-binding proteins such as streptavidin. Applications include DNA labeling techniques in fluorescence microscopy and flow cytometry, facilitating the visualization and analysis of nucleic acids in vitro.
| Physical Appearance | Solution |
| Storage | Store at -20°C or below |
| M.Wt | 947.8 (free acid) |
| Formula | C32H52N7O18P3S (free acid) |
| Synonyms | Biotin-16-Aminoallyl-2'-dUTP, Biotin-16-AA-dUTP |
| Chemical Name | lithium (E)-(5-(2,4-dioxo-5-(3-(4-(6-(5-(2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)hexanamido)butanamido)prop-1-en-1-yl)-3,4-dihydropyrimidin-1(2H)-yl)-3-hydroxytetrahydrofuran-2-yl)methyl triphosphate |
| SDF | Download SDF |
| Canonical SMILES | O=C(C(/C=C/CNC(CCCNC(CCCCCNC(CCCCC1C(NC(N2)=O)C2CS1)=O)=O)=O)=CN3C4CC(O)C(COP([O-])(OP([O-])(OP([O-])([O-])=O)=O)=O)O4)NC3=O.[Li+].[Li+].[Li+].[Li+] |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
Quality Control & MSDS
- View current batch:
-
Purity ≥90% by AX-HPLC
- COA (Certificate Of Analysis)
- MSDS (Material Safety Data Sheet)
Chemical structure
