Biotin-11-dUTP
Biotin-11-dUTP, a biotinylated deoxynucleotide triphosphate analogue, can be enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart deoxythymidine triphosphate (dTTP). The resulting biotin-labeled DNA/cDNA probes are then detected with streptavidin-horseradish peroxidase (HRP) conjugates, alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Biotin-11-dUTP is commonly used for PCR, nick translation, as well as reverse transcription. It is also suitable for apoptosis assays.
References:
1. Choi JH, Kim SY, Kim SK, et al. An Integrated Approach for Analysis of the DNA Damage Response in Mammalian Cells. Journal of Biological Chemistry, 2015, 290(48): 28812-28821.
2. Day PJ, Bevan IS, Gurney SJ, et al. Synthesis in vitro and application of biotinylated DNA probes for human papilloma virus type 16 by utilizing the polymerase chain reaction. Biochemical Journal, 1990, 267(1): 119-123.
| Physical Appearance | Solution |
| Storage | Store at -20°C or below |
| M.Wt | 887.44 |
| Cas No. | 1221498-88-9 |
| Formula | C28H44Li4N7O16P3S |
| Solubility | ≥100.7 mg/mL in H2O; ≥46.3 mg/mL in EtOH; ≥95.6 mg/mL in DMSO |
| Chemical Name | lithium (5-(4-amino-2-oxo-5-((E)-3-(6-(5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)hexanamido)prop-1-en-1-yl)-3,4-dihydropyrimidin-1(2H)-yl)-3-hydroxytetrahydrofuran-2-yl)methyl triphosphate |
| SDF | Download SDF |
| Canonical SMILES | O=C1N[C@]2([H])[C@]([C@H](CCCCC(NCCCCCC(NC/C=C/C3=CN(C(NC3N)=O)C4OC(COP(OP(OP([O-])([O-])=O)([O-])=O)([O-])=O)C(C4)O)=O)=O)SC2)([H])N1.[Li+].[Li+].[Li+].[Li+] |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
Quality Control & MSDS
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Purity = 99.89% by AX-HPLC
- COA (Certificate Of Analysis)
- MSDS (Material Safety Data Sheet)
Chemical structure
