Biotin-XX Tyramide Reagent

Biotin-XX Tyramide Reagent is used in tyramide signal amplification (TSA) for immunohistochemistry (IHC) and in situ hybridization (ISH): antibody-conjugated horseradish peroxidase (HRP) catalyzes localized covalent deposition of biotinylated tyramide onto adjacent targets, which is then detected via streptavidin conjugates for brightfield/fluorescence microscopy, aiding amplification of low-abundance protein or nucleic acid targets in histological/cytological samples, with fluorescence detection depending on the streptavidin-conjugated fluorophore's excitation and emission wavelengths. Biotin-XX-tyramide Reagent is a membrane-impermeant version of the proximity labeling probe biotin-tyramide(A8011). It is Biotin-XX-tyramide equipped with a long and polar polyamide linker. Unable to enter cells, it enables selective labeling of cell surface proteins rather than intracellular ones.