Biotin-XX Tyramide Reagent
Biotin-XX Tyramide Reagent is used in tyramide signal amplification (TSA) for immunohistochemistry (IHC) and in situ hybridization (ISH): antibody-conjugated horseradish peroxidase (HRP) catalyzes localized covalent deposition of biotinylated tyramide onto adjacent targets, which is then detected via streptavidin conjugates for brightfield/fluorescence microscopy, aiding amplification of low-abundance protein or nucleic acid targets in histological/cytological samples, with fluorescence detection depending on the streptavidin-conjugated fluorophore's excitation and emission wavelengths. Biotin-XX-tyramide Reagent is a membrane-impermeant version of the proximity labeling probe biotin-tyramide(A8011). It is Biotin-XX-tyramide equipped with a long and polar polyamide linker. Unable to enter cells, it enables selective labeling of cell surface proteins rather than intracellular ones.
- 1. Zora Chui-Kuen Chan, Cheng Qi, et al. "Revealing and mitigating the inhibitory effect of serotonin on HRP-mediated protein labelling." Sci Rep. 2024 Dec 30;14(1):32126. PMID: 39738643
- 2. Zora Chui-Kuen Chan, Cheng Qi, et al. "Serotonin-Induced Inhibition of HRP-Mediated Proximity Labelling." Research Square. 30 Dec, 2024
- 3. Pengzhen Dong, Yue Li, et al. "Fat body-derived cytokine Upd2 controls disciplined migration of tracheal stem cells in Drosophila." bioRxiv. November 12, 2024.
- 4. Carlos Pascual-Caro, Jaime de Juan-Sanz, et al. "Monitoring of activity-driven trafficking of endogenous synaptic proteins through proximity labeling." PLoS Biol. 2024 Oct 28;22(10):e3002860. PMID: 39466808
- 5. Ulku Cuhadar, Lorenzo Calzado-Reyes, et al. "Activity-driven synaptic translocation of LGI1 controls excitatory neurotransmission." Cell Rep. 2024 May 28;43(5):114186. PMID: 38700985
Physical Appearance | A solid |
Storage | Store at -20°C |
M.Wt | 589.79 |
Formula | C30H47N5O5S |
Synonyms | biotin-LC-LC-tyramide |
Solubility | ≥59 mg/mL in DMSO; insoluble in H2O; ≥14.1 mg/mL in EtOH with ultrasonic |
Chemical Name | N-(4-hydroxyphenethyl)-6-(6-(5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)hexanamido)hexanamide |
SDF | Download SDF |
Canonical SMILES | O=C1N[C@@]([H])([C@]([H])(CS2)N1)[C@@H]2CCCCC(NCCCCCC(NCCCCCC(NCCC3=CC=C(O)C=C3)=O)=O)=O |
Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
General tips | We do not recommend long-term storage for the solution, please use it up soon. |
Quality Control & MSDS
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Chemical structure
