Concanavalin A (Con A)-Biotin

Concanavalin A, Con A, derived from Canavalia ensiformis (Concanavalin), is a plant lectin protein (Mw = 104 kDa), each subunit can bind a Ca²⁺ and a Mn²⁺, contains a sugar-binding site, and when the metal ions bind to Con A, it can bind various glycoproteins, glycolipids and α-D-glucose and a-D-mannose moieties in sugars, Biotin, on the other hand, is a small molecule of water-soluble vitamins that has a very strong affinity for avidin. Concanavalin A-Biotin is a labeled protein that combines Concanavalin A and Biotin, and is widely used in biological research, particularly in immunohistochemistry (IHC), immunocytochemistry (ICC), and enzyme-linked immunosorbent assay (ELISA) to detect and quantify biomolecules containing specific sugar structures.