SwiftBlock? Blocking Buffer for Immunol Staining (Fast)

SwiftBlock? Blocking Buffer for Immunol Staining (Fast) is a next-generation, rapid and efficient blocking solution formulated in PBS. This product contains no serum, albumin, biotin, or detergents, but includes preservatives that do not affect HRP or AP activity. It is suitable for blocking samples and diluting primary or secondary antibodies in Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (IC), and similar experiments.

This blocking buffer is fast and efficient, typically requiring only 5–15 minutes. Compared to traditional blocking agents (e.g., BSA, non-fat dry milk, casein), it provides significantly stronger staining results. The absence of serum and albumin ensures an extremely high signal-to-noise ratio and very low background after blocking. It is compatible with horseradish peroxidase (HRP), alkaline phosphatase (AP), and biotin-labeled secondary antibodies. The product is convenient to use, requiring no additional reagents, and can be directly applied for sample blocking in immunostaining experiments.

This product is recommended for single use only, as repeated use often reduces blocking efficiency. However, for some primary antibodies with high signal-to-noise ratios (e.g., loading control antibodies), the buffer may be reused 2–3 times. Care should be taken to avoid mixing used blocking buffer with fresh, unused buffer.