SwiftBlock? Blocking Buffer (10X, Fast)

SwiftBlock? Blocking Buffer (10X, Fast) is a next-generation, rapid and efficient 10X concentrated blocking solution that can be diluted in PBS, TBS, or other appropriate buffers prior to use. This product contains no serum, albumin, biotin, or detergents, but includes preservatives that do not affect HRP or AP activity. It is suitable for blocking membranes or samples, as well as for diluting primary and secondary antibodies, in applications such as Western blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), and Immunocytochemistry (IC).

This blocking buffer is fast and efficient, typically requiring only 5–15 minutes of blocking time. Compared to traditional blocking agents (e.g., BSA, non-fat dry milk, casein), it provides a superior signal-to-noise ratio. The absence of serum and albumin ensures an extremely high signal-to-noise ratio and very low background after blocking. It is compatible with horseradish peroxidase (HRP), alkaline phosphatase (AP), and biotin-labeled secondary antibodies. The 10X concentrated solution offers flexible usage: it can be diluted to 1X with PBS, TBS, or other suitable buffers for experiments, and detergents (e.g., Tween-20 or Triton X-100) can be added to a final concentration of 0.05%–0.1% as needed for blocking or antibody dilution.

This product is recommended for single use only, as repeated use often reduces blocking efficiency. However, for some primary antibodies with high signal-to-noise ratios (e.g., loading control antibodies), the buffer may be reused 2–3 times. Care should be taken to avoid mixing used blocking buffer with fresh, unused buffer.