Biotin-tyramide
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Biotin-tyramide is used for tyramide signal amplification (TSA) which is a powerful, patented technology that significantly enhances both chromogenic and fluorescent signals. TSA is easily integrated into standard nonradioactive in situ hybridization (ISH) or IHC protocols.
TSA is an enzyme-mediated detection method that uses horseradish peroxidase (HRP) to catalyze the deposition of a fluorophore-labeled tyramide amplification reagent onto tissue sections or cell preparation surfaces that have been previously blocked with proteins.
Biotin-tyramide use horseradish peroxidase (HRP) to catalyze covalent deposition of biotin labels directly adjacent to the immobilized enzyme. The labeling reaction is quick (less than 10 minutes) and deposited labels can be detected with streptavidin conjugates for imaging in brightfield or fluorescence microscopy.
- 1. Clemens AM, Lenschow C, et al. "Estrus-Cycle Regulation of Cortical Inhibition." Curr Biol. 2019 Feb 18;29(4):605-615.e6. PMID:30744972
- 2. Liu P, Huang J, et al. "Mammalian mitochondrial RNAs are degraded in the mitochondrial intermembrane space by RNASET2." Protein Cell. 2017 Jul 20. PMID:28730546
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 363.47 |
| Cas No. | 41994-02-9 |
| Formula | C18H25N3O3S |
| Synonyms | Biotin-Phenol,Biotin Phenol |
| Solubility | insoluble in H2O; ≥100.2 mg/mL in DMSO; ≥8.18 mg/mL in EtOH with ultrasonic |
| Chemical Name | N-(4-hydroxyphenethyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide |
| SDF | Download SDF |
| Canonical SMILES | O=C1N[[email protected]@]([H])([[email protected]]([H])(CS2)N1)[[email protected]@H]2CCCCC(NCCC3=CC=C(O)C=C3)=O |
| Shipping Condition | Evaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request. |
| General tips | For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months. |
Quality Control & MSDS
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Chemical structure
Related Biological Data
Related Biological Data
