Biotin-tyramide
Catalog No.
A8011
Biotin-tyramide
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Biotin-tyramide is a biotinylation reagent specifically designed for tyramide signal amplification (TSA) methodologies in biological imaging and detection. TSA is an enzyme-mediated amplification procedure where horseradish peroxidase (HRP), conjugated to target-specific antibodies, catalyzes the deposition of biotin-labeled tyramide onto nearby protein residues in fixed cells or tissue sections. The reaction deposites biotin moieties precisely at detection sites. These biotin deposits can subsequently be identified via streptavidin-conjugated detection systems.The localization of deposition around HRP leads to good resolution. Suitable for either fluorescence or chromogenic detection. Common TSA applications include immunohistochemistry (IHC) and in situ hybridization (ISH).
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| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 363.47 |
| Cas No. | 41994-02-9 |
| Formula | C18H25N3O3S |
| Synonyms | Biotin-Phenol,Biotin Phenol |
| Solubility | insoluble in H2O; ≥100.2 mg/mL in DMSO; ≥8.18 mg/mL in EtOH with ultrasonic |
| Chemical Name | N-(4-hydroxyphenethyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide |
| SDF | Download SDF |
| Canonical SMILES | O=C1N[C@@]([H])([C@]([H])(CS2)N1)[C@@H]2CCCCC(NCCC3=CC=C(O)C=C3)=O |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
Quality Control & MSDS
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