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Catalog No.
Reagent used for tyramide signal amplification (TSA)
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Biotin-tyramide is used for tyramide signal amplification (TSA) which is a powerful, patented technology that significantly enhances both chromogenic and fluorescent signals. TSA is easily integrated into standard nonradioactive in situ hybridization (ISH) or IHC protocols.

TSA is an enzyme-mediated detection method that uses horseradish peroxidase (HRP) to catalyze the deposition of a fluorophore-labeled tyramide amplification reagent onto tissue sections or cell preparation surfaces that have been previously blocked with proteins.

Biotin-tyramide use horseradish peroxidase (HRP) to catalyze covalent deposition of biotin labels directly adjacent to the immobilized enzyme. The labeling reaction is quick (less than 10 minutes) and deposited labels can be detected with streptavidin conjugates for imaging in brightfield or fluorescence microscopy.

Product Citation

Chemical Properties

Physical AppearanceA solid
StorageStore at -20°C
Cas No.41994-02-9
SynonymsBiotin-Phenol,Biotin Phenol
Solubilityinsoluble in H2O; ≥100.2 mg/mL in DMSO; ≥8.18 mg/mL in EtOH with ultrasonic
Chemical NameN-(4-hydroxyphenethyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide
SDFDownload SDF
Canonical SMILESO=C1N[[email protected]@]([H])([[email protected]]([H])(CS2)N1)[[email protected]@H]2CCCCC(NCCC3=CC=C(O)C=C3)=O
Shipping ConditionEvaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.
General tipsFor obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.

Quality Control

Quality Control & MSDS

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Chemical structure


Related Biological Data


Related Biological Data