Cy3 TSA Fluorescence System Kit

Our Tyramide Signal Amplification (TSA) system can detect low-abundance targets in fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC), and in situ hybridization (ISH) techniques, enhancing signal sensitivity up to 100-fold.

The TSA fluorescence kits utilize horseradish peroxidase (HRP) to directly catalyze the covalent deposition of fluorescent groups near the immobilized enzyme. The labeling process is rapid (under 10 min), and can be directly visualized through standard or confocal microscope. TSA fluorescence kits can be used in combination with anti-fluorescence protocols.

TSA technology is used for bright field microscopy of enzyme conjugates and suitable chromogenic substrates. The use of TSA reagents results in a significant increase in sensitivity compared to standard assays while maintaining stable specificity and resolution. In addition, TSA reagents can significantly reduce the consumption of primary antibodies or probes. The fluorescent labeling signal of this kit (K1051) is Cyanine 3, which can be detected the signal by microscopy at the excitation and emission wavelengths (550 nm/570 nm).

Schematic diagram of tyramide signal amplification system

Label the cell or tissue sample with the primary and secondary antibodies using conventional methods. The horseradish peroxidase (HRP) conjugated to the second antibody catalyzes the conversion of the labeled tyramide to a reactive radical, and the tyramide radical is covalently bound to a nearby tyrosine residue to provide a high-density label.