Fluorescein TSA Fluorescence System Kit

Our Tyramide Signal Amplification (TSA) system can detect low-abundance targets in fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC), and in situ hybridization (ISH) techniques, enhancing signal sensitivity up to 100-fold.

The TSA fluorescence kits utilize horseradish peroxidase (HRP) to directly catalyze the covalent deposition of fluorescent groups near the immobilized enzyme. The labeling process is rapid (under 10 min), and can be directly visualized through standard or confocal microscope.

TSA fluorescence kits can be used in combination with anti-fluorescence protocols.TSA technology is suitable for brightfield microscopy applications with enzyme conjugates and appropriate chromogenic substrates.

This kit provides fluorescein-based fluorescent labels with excitation and emission wavelengths at 494 nm and 517 nm, respectively, detectable via microscope.

Schematic diagram of tyramide signal amplification system

Label the cell or tissue sample with the primary and secondary antibodies using conventional methods. The horseradish peroxidase (HRP) conjugated to the second antibody catalyzes the conversion of the labeled tyramide to a reactive radical, and the tyramide radical is covalently bound to a nearby tyrosine residue to provide a high-density label.