Fluorescein TSA Fluorescence System Kit

The Fluorescein TSA Fluorescence System uses tyramide signal amplification (TSA) to boost detection sensitivity in IHC, ICC, and ISH. HRP-linked secondary antibodies catalyze fluorescein-labeled tyramide into a reactive intermediate that covalently binds adjacent tyrosine residues, depositing high-density fluorescence around targets to amplify low-abundance molecule detection. Detectable via fluorescence microscopy (excitation 494 nm, emission 517 nm), it applies to analyzing proteins, nucleic acids, and other biomolecules in fixed cells and tissues.