HyperFluor™ 488 TSA Fluorescence System Kit

Tyramide Signal Amplification (TSA) technology is applied for detecting low-abundance targets in immunofluorescence (IF), immunohistochemistry (IHC), in situ hybridization (ISH), and other related assays in tissues and cells, increasing signal sensitivity up to approximately 100-fold. Fluorophore-labeled tyramide molecules are activated by horseradish peroxidase (HRP), which is conjugated to secondary antibodies, in the presence of hydrogen peroxide.

The fluorescence labeling signal of this kit is HF488 (excitation/emission: 495 nm/519 nm), which can be easily detected using a standard FITC filter set.

Our Tyramide Signal Amplification (TSA) product line also includes several additional kits: Kit K1050 with fluorescein signal (EX/EM: 494 nm/517 nm); Kit K1051 with Cyanine 3 signal (EX/EM: 550 nm/570 nm); and Kit K1052 with Cyanine 5 signal (EX/E: 648 nm/667 nm). Please select and purchase TSA kits with appropriate wavelengths according to your experimental requirements.

Schematic diagram of tyramide signal amplification system

Label the cell or tissue sample with the primary and secondary antibodies using conventional methods. The horseradish peroxidase (HRP) conjugated to the second antibody catalyzes the conversion of the labeled tyramide to a reactive radical, and the tyramide radical is covalently bound to a nearby tyrosine residue to provide a high-density label.