Biotin-11-dCTP
Biotin-11-dCTP is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dCTP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal substrate properties and thus labeling efficiency as well as an efficient detection of the Biotin moiety is ensured by a 11-atom linker attached to the C5 position of cytidine.
Recommended Biotin-11-dCTP/dCTP ratio for PCR and Nick Translation: 50% Biotin-11-dCTP/ 50% dCTP
Please note: The optimal final concentration of Biotin-11-dCTP may very depending on the application and assay conditions. For optimal product yields and high incorporation rates an individual optimization of the Biotin-11-dCTP/dCTP ratio is recommended.
- 1. Yongfeng Zhou, Hao Yu, et al. "Imprinted lncRNA KCNQ1OT1 regulates CDKN1C expression through promoter binding and chromatin folding in pigs." Gene. 2024 Sep 25:923:148590. PMID: 38772516
- 2. Yao Liu, Xiao Chen, et al. "Small cytosolic double-stranded DNA represses cyclic GMP-AMP synthase activation and induces autophagy." Cell Rep. 2023 Aug 29;42(8):112852. PMID: 37481718
| Physical Appearance | Solution |
| Storage | Store at -20°C or below |
| M.Wt | 859.67 (free acid) |
| Cas No. | 136632-30-9 |
| Formula | C28H44N7O16P3S (free acid) |
| Synonyms | Biotin-X-5-Propargylamino-dCTP |
| Chemical Name | γ-[N-(Biotin-6-amino-hexanoyl)]-5-propargylamino-2'-deoxycytidine-5'-triphosphate, Triethylammonium salt |
| SDF | Download SDF |
| Canonical SMILES | O=C1N([C@@H]2O[C@H](COP(OP(OP(O)(O)=O)(O)=O)(O)=O)[C@@H](O)C2)C=C(C#CCNC(CCCCCNC(CCCC[C@@]3([H])[C@](NC(N4)=O)([H])[C@]4([H])CS3)=O)=O)C(N)=N1 |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
Quality Control & MSDS
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Purity = 98.00%
- COA (Certificate Of Analysis)
- MSDS (Material Safety Data Sheet)
Chemical structure
